LOCUS BBa_K1124218 3122 bp DNA linear 10-May-2024 DEFINITION mCherry-luxI-tetR-aiiA (+d.term) ACCESSION BBa_K1124218 VERSION BBa_K1124218.1 FEATURES Location/Qualifiers RBS 1..12 /label=B0034 CDS 19..765 /label=mCherry-LVA misc_feature 19..21 /label=Start misc_feature 730..759 /label=ssrA degradation tag: LVA misc_feature 760..762 /label=Stop misc_feature 763..765 /label=Stop misc_feature 1..765 /label=BBa_K773003 misc_feature 778..781 /label=conserved RBS 774..785 /label=BBa_B0034 misc_feature 1371..1402 /label=LVA CDS 792..1370 /label=luxI misc_feature 792..1409 /label=BBa_C0061 misc_feature 1410..1434 /label=Help:Barcodes CDS 792..1409 /label=BBa_C0061 misc_feature 774..1434 /label=BBa_C0261 RBS 1443..1453 /label=RBS-4\Weaker RBS 1449..1452 /label=RBS misc_feature 1443..1453 /label=BBa_B0033 RBS 1443..1453 /label=BBa_B0033 CDS 1463..2079 /label=tetR misc_feature 2080..2113 /label=SsrA misc_feature 2120..2144 /label=Help:Barcodes CDS 1460..2119 /label=BBa_C0040 misc_feature 1443..2144 /label=BBa_S0107 RBS 2153..2165 /label=RBS-3\Weak RBS 2159..2162 /label=RBS misc_feature 2153..2165 /label=BBa_B0032 RBS 2153..2165 /label=BBa_B0032 misc_feature 2172..2174 /label=start misc_feature 2955..2960 /label=2 misc_feature 2922..2954 /label=LVA CDS 2172..2921 /label=aiiA misc_feature 2172..2960 /label=BBa_C0060 misc_feature 2961..2985 /label=Help:Barcodes CDS 2172..2960 /label=BBa_C0060 stem_loop 3005..3048 /label=stem_loop misc_feature 2994..3073 /label=BBa_B0010 terminator 2994..3073 /label=BBa_B0010 stem_loop 3089..3108 /label=T7 TE misc_feature 3115..3115 /label=stop polyA_site 3109..3122 /label=polya misc_feature 3082..3122 /label=BBa_B0012 terminator 3082..3122 /label=BBa_B0012 terminator 2994..3122 /label=BBa_B0015 misc_feature 2153..3122 /label=BBa_K516022 ORIGIN 1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 721 tacaaggcag caaacgacga aaactacgct ctagtagctt aataatacta gagaaagagg 781 agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg 841 agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact 901 tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt 961 atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg 1021 attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc 1081 ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact 1141 ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag 1201 gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta 1261 aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg 1321 ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg 1381 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 1441 agtcacacag gactactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 1501 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 1561 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 1621 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 1681 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 1741 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 1801 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 1861 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 1921 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 1981 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 2041 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agtcacacag 2161 gaaagtacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg 2221 atcattcgtc tgttaatagt acattaacac caggagaatt attagactta ccggtttggt 2281 gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg 2341 cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa 2401 tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc 2461 ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta 2521 taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat 2581 atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag 2641 tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat 2701 taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag 2761 agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa 2821 ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata 2881 tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact 2941 acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca 3001 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3061 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3121 ta //