LOCUS       BBa_K1124222            1743 bp    DNA     linear       19-May-2024
DEFINITION  mCherry-aiiA (+d.term)
ACCESSION   BBa_K1124222
VERSION     BBa_K1124222.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=B0034
     CDS             19..765
                     /label=mCherry-LVA
     misc_feature    19..21
                     /label=Start
     misc_feature    730..759
                     /label=ssrA degradation tag: LVA
     misc_feature    760..762
                     /label=Stop
     misc_feature    763..765
                     /label=Stop
     misc_feature    1..765
                     /label=BBa_K773003
     RBS             774..786
                     /label=RBS-3\Weak
     RBS             780..783
                     /label=RBS
     misc_feature    774..786
                     /label=BBa_B0032
     RBS             774..786
                     /label=BBa_B0032
     misc_feature    793..795
                     /label=start
     misc_feature    1576..1581
                     /label=2
     misc_feature    1543..1575
                     /label=LVA
     CDS             793..1542
                     /label=aiiA
     misc_feature    793..1581
                     /label=BBa_C0060
     misc_feature    1582..1606
                     /label=Help:Barcodes
     CDS             793..1581
                     /label=BBa_C0060
     stem_loop       1626..1669
                     /label=stem_loop
     misc_feature    1615..1694
                     /label=BBa_B0010
     terminator      1615..1694
                     /label=BBa_B0010
     stem_loop       1710..1729
                     /label=T7 TE
     misc_feature    1736..1736
                     /label=stop
     polyA_site      1730..1743
                     /label=polya
     misc_feature    1703..1743
                     /label=BBa_B0012
     terminator      1703..1743
                     /label=BBa_B0012
     terminator      1615..1743
                     /label=BBa_B0015
     misc_feature    774..1743
                     /label=BBa_K516022
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaaggcag caaacgacga aaactacgct ctagtagctt aataatacta gagtcacaca
      781 ggaaagtact agatgacagt aaagaagctt tatttcgtcc cagcaggtcg ttgtatgttg
      841 gatcattcgt ctgttaatag tacattaaca ccaggagaat tattagactt accggtttgg
      901 tgttatcttt tggagactga agaaggacct attttagtag atacaggtat gccagaaagt
      961 gcagttaata atgaaggtct ttttaacggt acatttgtcg aagggcaggt tttaccgaaa
     1021 atgactgaag aagatagaat cgtgaatatt ttaaaacggg ttggttatga gccggaagac
     1081 cttctttata ttattagttc tcacttgcat tttgatcatg caggaggaaa tggcgctttt
     1141 ataaatacac caatcattgt acagcgtgct gaatatgagg cggcgcagca tagcgaagaa
     1201 tatttgaaag aatgtatatt gccgaattta aactacaaaa tcattgaagg tgattatgaa
     1261 gtcgtaccag gagttcaatt attgcataca ccaggccata ctccagggca tcaatcgcta
     1321 ttaattgaga cagaaaaatc cggtcctgta ttattaacga ttgatgcatc gtatacgaaa
     1381 gagaattttg aaaatgaagt gccatttgcg ggatttgatt cagaattagc tttatcttca
     1441 attaaacgtt taaaagaagt ggtgatgaaa gagaagccga ttgttttctt tggacatgat
     1501 atagagcagg aaaggggatg taaagtgttc cctgaatata tagctgcaaa cgacgaaaac
     1561 tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
     1621 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1681 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1741 ata
//