LOCUS BBa_K1127016 3708 bp DNA linear 21-May-2024 DEFINITION Gold and QS ultra complex ACCESSION BBa_K1127016 VERSION BBa_K1127016.1 FEATURES Location/Qualifiers promoter 1..39 /label=PgolTS misc_feature 40..45 /label=SacII RBS 46..57 /label=rbs CDS 78..263 /label=lacZ-alpha misc_feature 265..271 /label=SandI misc_feature 64..77 /label=GFP wat? RBS 272..283 /label=rbs CDS 290..754 /label=GolS misc_feature 1..754 /label=BBa_K1127008 RBS 763..775 /label=RBS-3\Weak RBS 769..772 /label=RBS misc_feature 763..775 /label=BBa_B0032 RBS 763..775 /label=BBa_B0032 misc_feature 782..784 /label=start misc_feature 1565..1570 /label=2 misc_feature 1532..1564 /label=LVA CDS 782..1531 /label=aiiA misc_feature 782..1570 /label=BBa_C0060 misc_feature 1571..1595 /label=Help:Barcodes CDS 782..1570 /label=BBa_C0060 stem_loop 1615..1658 /label=stem_loop misc_feature 1604..1683 /label=BBa_B0010 terminator 1604..1683 /label=BBa_B0010 stem_loop 1699..1718 /label=T7 TE misc_feature 1725..1725 /label=stop polyA_site 1719..1732 /label=polya misc_feature 1692..1732 /label=BBa_B0012 terminator 1692..1732 /label=BBa_B0012 terminator 1604..1732 /label=BBa_B0015 misc_feature 763..1732 /label=BBa_K516022 promoter 1741..1775 /label=BBa_J23100 misc_feature 1788..1791 /label=conserved RBS 1784..1795 /label=BBa_B0034 misc_feature 1802..1803 /label=prefix misc_feature 1975..1975 /label=T misc_feature 2293..2293 /label=A CDS 1802..2551 /label=luxR misc_feature 1802..2557 /label=BBa_C0062 misc_feature 2558..2582 /label=Help:Barcodes CDS 1802..2557 /label=BBa_C0062 stem_loop 2602..2645 /label=stem_loop misc_feature 2591..2670 /label=BBa_B0010 terminator 2591..2670 /label=BBa_B0010 stem_loop 2686..2705 /label=T7 TE misc_feature 2712..2712 /label=stop polyA_site 2706..2719 /label=polya misc_feature 2679..2719 /label=BBa_B0012 terminator 2679..2719 /label=BBa_B0012 misc_feature 1784..2719 /label=BBa_I0462 misc_feature 2728..2902 /label=BBa_K1127010 misc_feature 2915..2918 /label=conserved RBS 2911..2922 /label=BBa_B0034 misc_feature 3508..3539 /label=LVA CDS 2929..3507 /label=luxI misc_feature 2929..3546 /label=BBa_C0061 misc_feature 3547..3571 /label=Help:Barcodes CDS 2929..3546 /label=BBa_C0061 stem_loop 3591..3634 /label=stem_loop misc_feature 3580..3659 /label=BBa_B0010 terminator 3580..3659 /label=BBa_B0010 stem_loop 3675..3694 /label=T7 TE misc_feature 3701..3701 /label=stop polyA_site 3695..3708 /label=polya misc_feature 3668..3708 /label=BBa_B0012 terminator 3668..3708 /label=BBa_B0012 terminator 3580..3708 /label=BBa_B0015 misc_feature 2911..3708 /label=BBa_K805016 ORIGIN 1 cttgaccttc ccacaatggc aagctttagg ctttctgatc cgcggaaaga ggagaaatac 61 tagatgaact atacaaaatg accatgatta cggattcact ggccgtcgtt ttacaacgtc 121 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 181 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 241 tgaatggcga atggcgctaa taaggggtcc caaagaggag aaaactagaa tgaacatcgg 301 taaagctgct aaagcttcta aagtttctgc taaaatgatc cgttactacg aacagatcgg 361 tctgatcccg gctgcttctc gtaccgactc tggttaccgt gcttacaccc aggctgacgt 421 taaccagctg cacttcatcc gtcgtgctcg tgacctgggt ttctctgttg ctgaaatctc 481 tgacctgctg aacctgtgga acaaccagtc tcgtcagtct gctgacgtta aacgtctggc 541 tcagacccac atcgacgaac tggaccgtcg tatccagaac atgcagcaca tggctcagac 601 cctgaaagct ctgatccact gctgcgctgg tgacgctctg ccggactgcc cgatcctgca 661 caccctgggt cagccggacg actctgaacc ggaagctcgt accggtgctg ttctgcgtcg 721 tccgcgtcgt cacggtctgg ctaaacgtct gtaatactag agtcacacag gaaagtacta 781 gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg atcattcgtc 841 tgttaatagt acattaacac caggagaatt attagactta ccggtttggt gttatctttt 901 ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg cagttaataa 961 tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa tgactgaaga 1021 agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc ttctttatat 1081 tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta taaatacacc 1141 aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat atttgaaaga 1201 atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag tcgtaccagg 1261 agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat taattgagac 1321 agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag agaattttga 1381 aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa ttaaacgttt 1441 aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata tagagcagga 1501 aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact acgctttagt 1561 agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa 1621 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1681 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 1741 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 1801 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 1861 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 1921 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 1981 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 2041 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 2101 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 2161 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 2221 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 2281 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 2341 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 2401 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 2461 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 2521 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 2581 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2641 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2701 gggcctttct gcgtttatat actagagaac ctgtaggatc gtacaggttt acttgtgagc 2761 ggataacaat atagtgtgtg gaattgtgag cggataacaa tttactagag acccaaatcc 2821 aggaggtgaa tctagtaggt ggttaatgaa aattaactta cttactagaa atatctctaa 2881 aaagccagat tattaatccg gctactagag aaagaggaga aatactagat gactataatg 2941 ataaaaaaat cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt 3001 cgttatcaag tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa 3061 tcagatgagt atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat 3121 gtaagtggat gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt 3181 cctgaattgc ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt 3241 tttgctgtag gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa 3301 ctatttgaag ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta 3361 acatcaacag caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga 3421 gacaaagaaa ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat 3481 gaacagttta aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct 3541 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 3601 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3661 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //