His

BBa_K112703 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112703

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:18

His affinity tag

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K112703_sequence (Version 1)

Description

This his tag can be fused to the N terminus of a protein.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

Construction of His-Tag> basic part K112703



Mix dv009/dv010                        (41bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+41, L)

Product is pBca1256-K112703



dv009	Forward Biobricking of His-Tag>

cgataGAATTCatgAGATCTatgCATCATCATCATCATCATGGATCCtaacg



dv010   Reverse Biobricking of His-Tag>

cgttaGGATCCATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112703/1

Member of these Collections

• //proteindomain/affinity
• //proteindomain/head
• iGEM Parts Registry