BBa_K112703
1
His
His affinity tag
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of His-Tag> basic part K112703
Mix dv009/dv010 (41bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+41, L)
Product is pBca1256-K112703
-----------------------------------------------
dv009 Forward Biobricking of His-Tag>
cgataGAATTCatgAGATCTatgCATCATCATCATCATCATGGATCCtaacg
dv010 Reverse Biobricking of His-Tag>
cgttaGGATCCATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
</pre>
This his tag can be fused to the N terminus of a protein.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112703_sequence
1
atgcatcatcatcatcatcat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z