S tag

BBa_K112704 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K112704
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:18



    • Details

    Types
    DnaRegion

    Roles
    tag

    Tag

    Sequences BBa_K112704_sequence (Version 1)

    Description

    This S-tag can be added after a protein part.

    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Source


    
Construction of 

    
Wobble PCR of dv011/dv012		(60 bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256			(EcoRI/BamHI)
    
Product is pBca1256-K112704
    


    
dv011		Forward Biobricking of 
cgataGAATTCatgAGATCTaaagaaaccgctgctgctaaattcgaacgcc
    

    
dv012		Reverse Biobricking of 
cgttaGGATCCttagctgtccatgtgctggcgttcgaatttagcagc


    igem#experience
    None
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K112704/1
     

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