Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jin Huh
Date created: 2008-10-31 12:00:00
Date modified: 2015-05-08 01:09:19
Enterobacteria phage T4 Lysis Device - no promoter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K112808_sequence (Version 1) |
Description
This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Various promoters can be installed with this device to control the lysis of bacteria. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.Notes
[Pcon].[Antiholin].[terminator] was assembled first, and then assembled with [T4 holin].[terminator]; Constitutive expression of antiholin is required before assembling holin.Source
Pubmed Accession Number: NC_000866Enterobacteria phage T4
| Access | Instance | Definition |
| public public public public public public public | BBa_K112805 BBa_K112806 BBa_B0010 BBa_B0012 BBa_J23116 BBa_K112807 BBa_B0010 | BBa_K112805 BBa_K112806 BBa_B0010 BBa_B0012 BBa_J23116 BBa_K112807 BBa_B0010 |