LOCUS       BBa_K1129016            1358 bp    DNA     linear       18-May-2024
DEFINITION  CaXMT1 under pTET constitutive promoter
ACCESSION   BBa_K1129016
VERSION     BBa_K1129016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..69
                     /label=Consensus sequence
     CDS             70..1185
                     /label=CaXMT1
     misc_feature    70..72
                     /label=Start codon
     misc_feature    1186..1215
                     /label=Strep Tag
     misc_feature    1216..1221
                     /label=Stop codon
     CDS             64..1221
                     /label=BBa_K801070
     stem_loop       1241..1284
                     /label=stem_loop
     misc_feature    1230..1309
                     /label=BBa_B0010
     terminator      1230..1309
                     /label=BBa_B0010
     stem_loop       1325..1344
                     /label=T7 TE
     misc_feature    1351..1351
                     /label=stop
     polyA_site      1345..1358
                     /label=polya
     misc_feature    1318..1358
                     /label=BBa_B0012
     terminator      1318..1358
                     /label=BBa_B0012
     terminator      1230..1358
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gagtacacaa tgtctttaca agaagtcttg agaatgaacg gtggtgaagg tgatacttct
      121 tacgctaaaa actccgccta caatcaattg gttttggcta aagttaagcc agtcttggaa
      181 caatgcgtca gagaattatt gagagctaac ttgccaaaca tcaacaagtg cattaaggtt
      241 gctgatttgg gttgtgcttc tggtccaaat actttgttga ctgttagaga catcgtccaa
      301 tccattgata aggttggtca agaaaagaag aacgaattgg aaagaccaac catccaaatt
      361 ttcttgaacg acttgttccc aaacgacttc aactctgttt ttaagttgtt gccatccttc
      421 tacagaaagt tggaaaaaga aaacggtaga aagatcggtt cctgtttgat tggtgctatg
      481 ccaggttctt tctactccag attatttcct gaagaatcca tgcatttctt gcactcttgt
      541 tattgcttgc aatggttgtc tcaagttcca tctggtttgg ttactgaatt gggtatttct
      601 accaacaagg gttccatcta ctcttctaaa gcttcaagat tgccagttca aaaggcctac
      661 ttggatcaat tcactaagga tttcaccacc tttttgagaa tccactccga agaattattc
      721 tcccacggta gaatgttgtt gacctgtata tgtaagggtg ttgaattgga tgctagaaac
      781 gccattgatt tgttggaaat ggctatcaac gatttggttg ttgaaggtca cttagaagaa
      841 gaaaagttgg actctttcaa cttgccagtt tacattccat ctgccgaaga agttaagtgc
      901 atcgttgaag aagaaggttc cttcgaaatc ttgtacttgg aaactttcaa ggtcttgtac
      961 gatgccggtt tctctattga tgatgaacat attaaggccg aatacgttgc ctcttctgtt
     1021 agagctgttt acgaacctat tttggcttct catttcggtg aagccattat cccagatatc
     1081 tttcatagat ttgctaagca cgctgctaag gttttgccat tgggtaaagg tttttacaac
     1141 aacttgatca tctccttggc caaaaagcca gaaaagtctg atgtttctgc ttggtcacat
     1201 ccacaattcg aaaagtgatg atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1261 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1321 cactggctca ccttcgggtg ggcctttctg cgtttata
//