LOCUS BBa_K1129019 4144 bp DNA linear 18-May-2024 DEFINITION Complete caffeine synthesis pathway under pTET constitutive promoter ACCESSION BBa_K1129019 VERSION BBa_K1129019.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23118 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 64..69 /label=Consensus sequence CDS 70..1185 /label=CaXMT1 misc_feature 70..72 /label=Start codon misc_feature 1186..1215 /label=Strep Tag misc_feature 1216..1221 /label=Stop codon CDS 64..1221 /label=BBa_K801070 stem_loop 1241..1284 /label=stem_loop misc_feature 1230..1309 /label=BBa_B0010 terminator 1230..1309 /label=BBa_B0010 stem_loop 1325..1344 /label=T7 TE misc_feature 1351..1351 /label=stop polyA_site 1345..1358 /label=polya misc_feature 1318..1358 /label=BBa_B0012 terminator 1318..1358 /label=BBa_B0012 terminator 1230..1358 /label=BBa_B0015 promoter 1367..1401 /label=BBa_J23118 misc_feature 1414..1417 /label=conserved RBS 1410..1421 /label=BBa_B0034 CDS 1436..2569 /label=CaMXMT1 misc_feature 1430..1435 /label=Consensus sequence misc_feature 2570..2599 /label=Strep Tag misc_feature 1436..1438 /label=Start codon misc_feature 2600..2605 /label=Stop codon CDS 1430..2605 /label=BBa_K801071 stem_loop 2625..2668 /label=stem_loop misc_feature 2614..2693 /label=BBa_B0010 terminator 2614..2693 /label=BBa_B0010 stem_loop 2709..2728 /label=T7 TE misc_feature 2735..2735 /label=stop polyA_site 2729..2742 /label=polya misc_feature 2702..2742 /label=BBa_B0012 terminator 2702..2742 /label=BBa_B0012 terminator 2614..2742 /label=BBa_B0015 promoter 2751..2785 /label=BBa_J23118 misc_feature 2798..2801 /label=conserved RBS 2794..2805 /label=BBa_B0034 misc_feature 2814..2819 /label=Consensus sequence CDS 2820..3971 /label=CaDXMT1 misc_feature 3972..4001 /label=Strep Tag misc_feature 4002..4007 /label=Stop codon misc_feature 2820..2822 /label=Start codon CDS 2814..4007 /label=BBa_K801072 stem_loop 4027..4070 /label=stem_loop misc_feature 4016..4095 /label=BBa_B0010 terminator 4016..4095 /label=BBa_B0010 stem_loop 4111..4130 /label=T7 TE misc_feature 4137..4137 /label=stop polyA_site 4131..4144 /label=polya misc_feature 4104..4144 /label=BBa_B0012 terminator 4104..4144 /label=BBa_B0012 terminator 4016..4144 /label=BBa_B0015 ORIGIN 1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta 61 gagtacacaa tgtctttaca agaagtcttg agaatgaacg gtggtgaagg tgatacttct 121 tacgctaaaa actccgccta caatcaattg gttttggcta aagttaagcc agtcttggaa 181 caatgcgtca gagaattatt gagagctaac ttgccaaaca tcaacaagtg cattaaggtt 241 gctgatttgg gttgtgcttc tggtccaaat actttgttga ctgttagaga catcgtccaa 301 tccattgata aggttggtca agaaaagaag aacgaattgg aaagaccaac catccaaatt 361 ttcttgaacg acttgttccc aaacgacttc aactctgttt ttaagttgtt gccatccttc 421 tacagaaagt tggaaaaaga aaacggtaga aagatcggtt cctgtttgat tggtgctatg 481 ccaggttctt tctactccag attatttcct gaagaatcca tgcatttctt gcactcttgt 541 tattgcttgc aatggttgtc tcaagttcca tctggtttgg ttactgaatt gggtatttct 601 accaacaagg gttccatcta ctcttctaaa gcttcaagat tgccagttca aaaggcctac 661 ttggatcaat tcactaagga tttcaccacc tttttgagaa tccactccga agaattattc 721 tcccacggta gaatgttgtt gacctgtata tgtaagggtg ttgaattgga tgctagaaac 781 gccattgatt tgttggaaat ggctatcaac gatttggttg ttgaaggtca cttagaagaa 841 gaaaagttgg actctttcaa cttgccagtt tacattccat ctgccgaaga agttaagtgc 901 atcgttgaag aagaaggttc cttcgaaatc ttgtacttgg aaactttcaa ggtcttgtac 961 gatgccggtt tctctattga tgatgaacat attaaggccg aatacgttgc ctcttctgtt 1021 agagctgttt acgaacctat tttggcttct catttcggtg aagccattat cccagatatc 1081 tttcatagat ttgctaagca cgctgctaag gttttgccat tgggtaaagg tttttacaac 1141 aacttgatca tctccttggc caaaaagcca gaaaagtctg atgtttctgc ttggtcacat 1201 ccacaattcg aaaagtgatg atactagagc caggcatcaa ataaaacgaa aggctcagtc 1261 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1321 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cggctagctc 1381 agtcctaggt attgtgctag ctactagaga aagaggagaa atactagagt acacaatgtc 1441 tttacaagaa gtcttgcaca tgaacgaagg tgaaggtgat acttcttacg ctaagaatgc 1501 ttcttacaac ttggctttgg ctaaggttaa gccattcttg gaacaatgca tcagagaatt 1561 attgagagcc aacttgccaa acatcaacaa gtgtattaag gttgctgatt tgggttgtgc 1621 ttctggtcca aatactttgt tgactgttag agacatcgtc caatccattg ataaggttgg 1681 tcaagaagaa aagaacgaat tggaaagacc aaccatccaa attttcttga acgacttgtt 1741 ccaaaacgac ttcaactccg tttttaagtt gttgccatcc ttctacagaa agttggaaaa 1801 agaaaacggt agaaagatcg gttcctgctt gatttctgct atgccaggtt ctttttacgg 1861 tagattattc cctgaagaat ccatgcattt cttgcactct tgttactctg ttcactggtt 1921 gtctcaagtt ccatctggtt tggttattga attgggtatt ggtgctaaca agggttccat 1981 ctattcttct aaaggttgta gaccaccagt tcaaaaggct tacttggatc aattcactaa 2041 ggacttcacc actttcttga gaatccactc caaagaatta ttctccagag gtagaatgtt 2101 gttgacctgt atctgtaagg ttgacgaatt tgatgaacct aacccattgg atttgttgga 2161 tatggccatt aacgatttga tcgtcgaagg tttgttggaa gaagaaaagt tggactcctt 2221 caacattcca ttctttactc catctgccga agaagttaag tgcatcgttg aagaagaagg 2281 ttcttgcgaa atcttgtact tggaaacttt caaggctcat tacgatgctg ccttctctat 2341 tgatgatgat tacccagtta gatcccacga acaaatcaaa gctgaatacg ttgcctcctt 2401 gatcagatct gtttacgaac ctattttggc ctctcatttc ggtgaagcta ttatgccaga 2461 tttgtttcac agattggcta aacatgctgc taaggtttta cacatgggta agggttgtta 2521 caacaacttg attatctcct tggccaaaaa gccagaaaag tctgatgttt ctgcttggtc 2581 acatccacaa ttcgaaaagt gatgatacta gagccaggca tcaaataaaa cgaaaggctc 2641 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2701 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacggcta 2761 gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta gagtacacaa 2821 tgtctttaca agaagtcttg catatgaacg gtggtgaagg tgatacttct tacgctaaga 2881 actctttcta caacttgttc ttgatcagag tcaagccaat cttggaacaa tgcatccaag 2941 aattattgag agccaacttg ccaaacatca acaagtgtat taaggttgct gatttgggtt 3001 gtgcttctgg tccaaatact ttgttgactg ttagagacat cgtccaatcc attgataagg 3061 ttggtcaaga aaagaagaac gaattggaaa gaccaaccat ccaaattttc ttgaacgact 3121 tgttccaaaa cgacttcaac tccgttttta agtccttgcc atccttctac agaaagttgg 3181 aaaaagaaaa cggtagaaag atcggttcct gtttgattgg tgctatgcca ggttcttttt 3241 acggtagatt attccctgaa gaatccatgc atttcttgca ttcttgttac tgcttgcact 3301 ggttgtctca agttccatct ggtttggtta ctgaattggg tatttctgct aacaagggtt 3361 gcatctactc ttctaaagct tcaagaccac caattcaaaa ggcctacttg gatcaattca 3421 ctaaggattt caccactttc ttgagaatcc actccgaaga attgatcagt agaggtagaa 3481 tgttgttgac ctggatctgc aaagaagatg aatttgaaaa cccaaactcc atcgatttgt 3541 tggaaatgtc catcaacgat ttggttatcg aaggtcactt agaagaagaa aagttggact 3601 ctttcaacgt tccaatctat gctccatcta ccgaagaagt taagtgcatc gttgaagaag 3661 aaggttcctt cgaaatcttg tacttggaaa cctttaaggt tccatacgat gccggtttct 3721 ctatcgatga tgattatcaa ggtagatccc actctccagt ttcttgtgat gaacatgcta 3781 gagctgctca tgttgcttca gttgttagat ctattttcga acctatcgtt gcctctcatt 3841 tcggtgaagc tattatgcca gatttgtccc atagaattgc taagaatgct gccaaggttt 3901 tgagatctgg taagggtttt tacgactcct tgattatttc cttggccaaa aagccagaaa 3961 agtccgatgt ttctgcttgg tcacatccac aattcgaaaa gtgatgatac tagagccagg 4021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 4081 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 4141 tata //