LOCUS       BBa_K1129045            2209 bp    DNA     linear       18-May-2024
DEFINITION  Phenylalanine ammonia lyase under pTET constitutive promoter
ACCESSION   BBa_K1129045
VERSION     BBa_K1129045.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=Start codon
     misc_feature    1417..1417
                     /label=Mutation
     CDS             62..2209
                     /label=PAL
     misc_feature    1419..1421
                     /label=Deletion of Cys
     misc_feature    1425..1425
                     /label=Phe instead of Ser
     CDS             62..2209
                     /label=BBa_K801091
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatggcaccc tcgctcgact cgatctcgca ctcgttcgca aacggcgtcg catccgcaaa
      121 gcaggctgtc aatggcgcct cgaccaacct cgcagtcgca ggctcgcacc tgcccacaac
      181 ccaggtcacg caggtcgaca tcgtcgagaa gatgctcgcc gcgccgaccg actcgacgct
      241 cgaactcgac ggctactcgc tcaacctcgg agacgtcgtc tcggccgcga ggaagggcag
      301 gcctgtccgc gtcaaggaca gcgacgagat ccgctcaaag attgacaaat cggtcgagtt
      361 cttgcgctcg caactctcca tgagcgtcta cggcgccacg actggatttg gcggatccgc
      421 agacacccgc accgaggacg ccatctcgct ccagaaggct ctcctcgagc accagctctg
      481 cggtgttctc ccttcgtcgt tcgactcgtt ccgcctcggc cgcggtctcg agaactcgct
      541 tcccctcgag gttgttcgcg gcgccatgac aatccgcgtc aacagcttga cccgcggcca
      601 ctcggctgtc cgcctcgtcg tcctcgaggc gctcaccaac ttcctcaacc acggcatcac
      661 ccccatcgtc cccctccgcg gcaccatctc tgcgtcgggc gacctctctc ctctctccta
      721 cattgcagcg gccatcagcg gtcacccgga cagcaaggtg cacgtcgtcc acgagggcaa
      781 ggagaagatc ctgtacgccc gcgaggcgat ggcgctcttc aacctcgagc ccgtcgtcct
      841 cggcccgaag gaaggtctcg gtctcgtcaa cggcaccgcc gtctcagcat cgatggccac
      901 cctcgctctg cacgacgcac acatgctctc gctcctctcg cagtcgctca cggccatgac
      961 ggtcgaagcg atggtcggcc acgccggctc gttccacccc ttccttcacg acgtcacgcg
     1021 ccctcacccg acgcagatcg aagtcgcggg aaacatccgc aggctcctcg agggaagccg
     1081 ctttgctgtc caccatgagg aggaggtcaa ggtcaaggac gacgagggca ttctccgcca
     1141 ggaccgctac cccttgcgca cgtctcctca gtggctcggc ccgctcgtca gcgacctcat
     1201 tcacgcccac gccgtcctca ccatcgaggc cggccagtcg acgaccgaca accctctcat
     1261 cgacgtcggg aacaagactt cgcaccacgg cggcaatttc caggctgccg ctgtggccaa
     1321 caccatggag aagactcgcc tcgggctcgc ccagatcggc aagctcaact tcacgcagct
     1381 caccgagatg ctcaacgccg gcatgaaccg cggcctcccc tcctgcctcg cggccgaaga
     1441 cccctcgctc tcctaccact gcaagggcct cgacatcgcc gctgcggcgt acacctcgga
     1501 gttgggacac ctcgccaacc ctgtgacgac gcatgtccag ccggctgaga tggcgaacca
     1561 ggcggtcaac tcgcttgcgc tcatctcggc tcgtcgcacg accgagtcca acgacgtcct
     1621 ttctctcctc ctcgccaccc acctctactg cgttctccaa gccatcgact tgcgcgcgat
     1681 cgagttcgag ttcaagaagc agttcggccc agccatcgtc tcgctcatcg accagcactt
     1741 tggctccgcc atgaccggct cgaacctgcg cgacgagctc gtcgagaagg tgaacaagac
     1801 gctcgccaag cgcctcgagc agaccaactc gtacgacctc gtcccgcgct ggcacgacgc
     1861 cttctccttc gccgccggca ccgtcgtcga ggtcctctcg tcgacgtcgc tctcgctcgc
     1921 cgccgtcaac gcctggaagg tcgccgccgc cgagtcggcc atctcgctca cccgccaagt
     1981 ccgcgagacc ttctggtccg ccgcgtcgac ctcgtcgccc gcgctctcgt acctctcgcc
     2041 gcgcactcag atcctctacg ccttcgtccg cgaggagctt ggcgtcaagg cccgccgcgg
     2101 agacgtcttc ctcggcaagc aagaggtgac gatcggctcg aacgtctcca agatctacga
     2161 ggccatcaag tcgggcagga tcaacaacgt cctcctcaag atgctcgct
//