| Types || |
| Roles |
| Sequences || BBa_K113012_sequence (Version 1)|
DescriptionTwo T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
NotesIt is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
SourceThe sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').