LOCUS       BBa_K113015             1806 bp    DNA     linear       17-May-2024
DEFINITION  bidirectional intact T7 promoters->GFP/RFP
ACCESSION   BBa_K113015
VERSION     BBa_K113015.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     CDS             136..855
                     /label=GFP protein
     CDS             136..855
                     /label=BBa_E0040
     misc_feature    868..871
                     /label=conserved
     RBS             864..875
                     /label=BBa_B0034
     promoter        884..906
                     /label=BBa_I719005
     promoter        915..937
                     /label=BBa_I719005
     misc_feature    950..953
                     /label=conserved
     RBS             946..957
                     /label=BBa_B0034
     CDS             964..1638
                     /label=mrfp1
     misc_feature    1645..1669
                     /label=Help:Barcodes
     CDS             964..1669
                     /label=BBa_E1010
     stem_loop       1689..1732
                     /label=stem_loop
     misc_feature    1678..1757
                     /label=BBa_B0010
     terminator      1678..1757
                     /label=BBa_B0010
     stem_loop       1773..1792
                     /label=T7 TE
     misc_feature    1799..1799
                     /label=stop
     polyA_site      1793..1806
                     /label=polya
     misc_feature    1766..1806
                     /label=BBa_B0012
     terminator      1766..1806
                     /label=BBa_B0012
     terminator      1678..1806
                     /label=BBa_B0015
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      181 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      241 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      301 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      361 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      421 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      481 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      541 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      601 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      661 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      721 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      781 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      841 ctatacaaat aataatacta gagaaagagg agaaatacta gagtaatacg actcactata
      901 gggagatact agagtaatac gactcactat agggagatac tagagaaaga ggagaaatac
      961 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
     1021 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
     1081 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
     1141 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
     1201 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
     1261 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
     1321 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
     1381 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
     1441 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
     1501 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
     1561 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
     1621 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
     1681 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1741 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1801 tttata
//