LOCUS       BBa_K113017             1789 bp    DNA     linear       04-May-2024
DEFINITION  more overlapping T7 promoter->GFP/RFP
ACCESSION   BBa_K113017
VERSION     BBa_K113017.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     CDS             136..855
                     /label=GFP protein
     CDS             136..855
                     /label=BBa_E0040
     misc_feature    868..871
                     /label=conserved
     RBS             864..875
                     /label=BBa_B0034
     promoter        887..904
                     /label=T7 promoter
     promoter        900..917
                     /label=T7 promoter
     promoter        884..920
                     /label=BBa_K113011
     misc_feature    933..936
                     /label=conserved
     RBS             929..940
                     /label=BBa_B0034
     CDS             947..1621
                     /label=mrfp1
     misc_feature    1628..1652
                     /label=Help:Barcodes
     CDS             947..1652
                     /label=BBa_E1010
     stem_loop       1672..1715
                     /label=stem_loop
     misc_feature    1661..1740
                     /label=BBa_B0010
     terminator      1661..1740
                     /label=BBa_B0010
     stem_loop       1756..1775
                     /label=T7 TE
     misc_feature    1782..1782
                     /label=stop
     polyA_site      1776..1789
                     /label=polya
     misc_feature    1749..1789
                     /label=BBa_B0012
     terminator      1749..1789
                     /label=BBa_B0012
     terminator      1661..1789
                     /label=BBa_B0015
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      181 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      241 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      301 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      361 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      421 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      481 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      541 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      601 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      661 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      721 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      781 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      841 ctatacaaat aataatacta gagaaagagg agaaatacta gagcccctat agtgagtcgt
      901 actacgactc actatagggg tactagagaa agaggagaaa tactagatgg cttcctccga
      961 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
     1021 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
     1081 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
     1141 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
     1201 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
     1261 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
     1321 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
     1381 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
     1441 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
     1501 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
     1561 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
     1621 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
     1681 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1741 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//