LOCUS BBa_K113021 7322 bp DNA linear 18-May-2024 DEFINITION Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter ACCESSION BBa_K113021 VERSION BBa_K113021.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 138..2815 /label=BBa_I712069 promoter 3903..4033 /label=Pbad promoter 3866..3937 /label=PC misc_feature 2824..3902 /label=AraC promoter 2824..4033 /label=BBa_I0500 stem_loop 4053..4096 /label=stem_loop misc_feature 4042..4121 /label=BBa_B0010 terminator 4042..4121 /label=BBa_B0010 stem_loop 4137..4156 /label=T7 TE misc_feature 4163..4163 /label=stop polyA_site 4157..4170 /label=polya misc_feature 4130..4170 /label=BBa_B0012 terminator 4130..4170 /label=BBa_B0012 terminator 4042..4170 /label=BBa_B0015 CDS 4177..4896 /label=GFP protein CDS 4177..4896 /label=BBa_E0040 misc_feature 4909..4912 /label=conserved RBS 4905..4916 /label=BBa_B0034 CDS 4926..5542 /label=tetR misc_feature 5543..5576 /label=SsrA misc_feature 5583..5607 /label=Help:Barcodes CDS 4923..5607 /label=BBa_C0040 misc_feature 5620..5623 /label=conserved RBS 5616..5627 /label=BBa_B0034 misc_feature 5636..5678 /label=OR2 misc_feature 5643..5656 /label=OR2 misc_feature 5665..5678 /label=OR1 promoter 5636..5641 /label=-35 promoter 5659..5664 /label=-10 promoter 5637..5642 /label=-35 misc_feature 5670..5672 /label=start misc_feature 5636..5681 /label=BBa_R0052 promoter 5636..5681 /label=BBa_R0052 promoter 5693..5710 /label=T7 promoter promoter 5709..5726 /label=T7 promoter promoter 5690..5729 /label=BBa_K113010 misc_feature 5738..5756 /label=TetR 1 misc_feature 5738..5791 /label=BBa_R0040 promoter 5757..5762 /label=-35 misc_feature 5763..5781 /label=TetR 2 promoter 5780..5785 /label=-10 promoter 5738..5791 /label=BBa_R0040 misc_feature 5804..5807 /label=conserved RBS 5800..5811 /label=BBa_B0034 misc_feature 6448..6453 /label=2 CDS 5818..6453 /label=cI 434 CDS 5818..6453 /label=BBa_C0056 misc_feature 6466..6469 /label=conserved RBS 6462..6473 /label=BBa_B0034 CDS 6480..7154 /label=mrfp1 misc_feature 7161..7185 /label=Help:Barcodes CDS 6480..7185 /label=BBa_E1010 stem_loop 7205..7248 /label=stem_loop misc_feature 7194..7273 /label=BBa_B0010 terminator 7194..7273 /label=BBa_B0010 stem_loop 7289..7308 /label=T7 TE misc_feature 7315..7315 /label=stop polyA_site 7309..7322 /label=polya misc_feature 7282..7322 /label=BBa_B0012 terminator 7282..7322 /label=BBa_B0012 terminator 7194..7322 /label=BBa_B0015 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagcca ccatgaacac gattaacatc gctaagaacg acttctctga 181 catcgaactg gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc 241 tcgcgaacag ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa 301 gatgtttgag cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct 361 catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa 421 agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc 481 cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac 541 cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg 601 tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg 661 cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa 721 gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg 781 agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa 841 tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc 901 tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt 961 tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc 1021 tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat 1081 gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa 1141 agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc 1201 tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc 1261 tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc 1321 tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc 1381 catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa 1441 cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg 1501 taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt 1561 tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc 1621 taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc 1681 gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc 1741 gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga 1801 ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat 1861 tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga 1921 agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac 1981 taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc 2041 agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga 2101 taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc 2161 tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt 2221 tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa 2281 gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt 2341 ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg 2401 tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa 2461 acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa 2521 gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc 2581 cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt 2641 tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt 2701 gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg 2761 tgacatctta gagtcggact tcgcgttcgc ggatccaaaa aagaagagaa aggtatacta 2821 gagttatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc 2881 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa 2941 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc 3001 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa 3061 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa 3121 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat 3181 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag 3241 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc 3301 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc 3361 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca 3421 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg 3481 gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc 3541 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa 3601 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa 3661 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc 3721 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta 3781 ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag 3841 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt 3901 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag 3961 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt 4021 tttttgggct agctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 4081 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 4141 caccttcggg tgggcctttc tgcgtttata tactagatgc gtaaaggaga agaacttttc 4201 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 4261 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 4321 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt 4381 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 4441 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 4501 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 4561 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 4621 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 4681 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 4741 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 4801 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 4861 attacacatg gcatggatga actatacaaa taataatact agagaaagag gagaaatact 4921 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg 4981 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta 5041 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt 5101 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac 5161 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac 5221 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt 5281 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt 5341 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa 5401 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc 5461 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac 5521 aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat 5581 aacactgata gtgctagtgt agatcactac tagagaaaga ggagaaatac tagagttgac 5641 aaacaagata cattgtatga aaatacaaga aagtttgttg atactagagc ccctatagtg 5701 agtcgtatta atacgactca ctataggggt actagagtcc ctatcagtga tagagattga 5761 catccctatc agtgatagag atactgagca ctactagaga aagaggagaa atactagatg 5821 agtatttctt ccagggtaaa aagcaaaaga atccagcttg gacttaacca ggctgaactt 5881 gctcaaaagg tggggactac ccagcagtct atagagcagc tcgaaaacgg taaaactaag 5941 cgaccacgct ttttaccaga acttgcgtca gctcttggcg taagtgttga ctggctgctc 6001 aatggcacct ctgattcgaa tgttagattt gttgggcacg ttgagcccaa agggaaatat 6061 ccattgatta gcatggttag agctggttcg tggtgtgaag cttgtgaacc ctacgatatc 6121 aaggacattg atgaatggta tgacagtgac gttaacttat taggcaatgg attctggctg 6181 aaggttgaag gtgattccat gacctcacct gtaggtcaaa gcatccctga aggtcatatg 6241 gtgttagtag atactggacg ggagccagtg aatggaagcc ttgttgtagc caaactgact 6301 gacgcgaacg aagcaacatt caagaaactg gtcatagatg gcggtcagaa gtacctgaaa 6361 ggcctgaatc cttcatggcc tatgactcct atcaacggaa actgcaagat tatcggtgtt 6421 gtcgtggaag cgagggtaaa attcgtataa taatactaga gaaagaggag aaatactaga 6481 tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt 6541 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta 6601 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc 6661 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg 6721 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag 6781 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca 6841 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca 6901 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa 6961 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct 7021 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca 7081 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc 7141 actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca 7201 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 7261 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 7321 ta //