LOCUS       BBa_K1132013            2465 bp    DNA     linear       01-Jun-2024
DEFINITION  heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and 
            tetracycline repressor (TetR) under const
ACCESSION   BBa_K1132013
VERSION     BBa_K1132013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    1..56
                     /label=BBa_K608003
     misc_feature    789..813
                     /label=Help:Barcodes
     CDS             63..813
                     /label=BBa_I15008
     RBS             822..836
                     /label=RBS-1\Strong
     RBS             829..833
                     /label=RBS
     misc_feature    822..836
                     /label=BBa_B0030
     RBS             822..836
                     /label=BBa_B0030
     CDS             843..1592
                     /label=PcyA
     misc_feature    1593..1617
                     /label=Help:Barcodes
     CDS             843..1617
                     /label=BBa_I15009
     misc_feature    822..1617
                     /label=BBa_K081017
     CDS             63..1617
                     /label=BBa_K1132011
     misc_feature    1630..1633
                     /label=conserved
     RBS             1626..1637
                     /label=BBa_B0034
     CDS             1647..2263
                     /label=tetR
     misc_feature    2264..2297
                     /label=SsrA
     misc_feature    2304..2328
                     /label=Help:Barcodes
     CDS             1644..2303
                     /label=BBa_C0040
     stem_loop       2348..2391
                     /label=stem_loop
     misc_feature    2337..2416
                     /label=BBa_B0010
     terminator      2337..2416
                     /label=BBa_B0010
     stem_loop       2432..2451
                     /label=T7 TE
     misc_feature    2458..2458
                     /label=stop
     polyA_site      2452..2465
                     /label=polya
     misc_feature    2425..2465
                     /label=BBa_B0012
     terminator      2425..2465
                     /label=BBa_B0012
     misc_feature    1626..2465
                     /label=BBa_P0440
     CDS             63..2465
                     /label=BBa_K1132012
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgagtgt caacttagct tcccagttgc gggaagggac gaaaaaatcc cactccatgg
      121 cggagaacgt cggctttgtc aaatgcttcc tcaagggcgt tgtcgagaaa aattcctacc
      181 gtaagctggt tggcaatctc tactttgtct acagtgccat ggaagaggaa atggcaaaat
      241 ttaaggacca tcccatcctc agccacattt acttccccga actcaaccgc aaacaaagcc
      301 tagagcaaga cctgcaattc tattacggct ccaactggcg gcaagaagtg aaaatttctg
      361 ccgctggcca agcctatgtg gaccgagtcc ggcaagtggc cgctacggcc cctgaattgt
      421 tggtggccca ttcctacacc cgttacctgg gggatctttc cggcggtcaa attctcaaga
      481 aaattgccca aaatgccatg aatctccacg atggtggcac agctttctat gaatttgccg
      541 acattgatga cgaaaaggct tttaaaaata cctaccgtca agctatgaat gatctgccca
      601 ttgaccaagc caccgccgaa cggattgtgg atgaagccaa tgacgccttt gccatgaaca
      661 tgaaaatgtt caacgaactt gaaggcaacc tgatcaaggc gatcggcatt atggtgttca
      721 acagcctcac ccgtcgccgc agtcaaggca gcaccgaagt tggcctcgcc acctccgaag
      781 gctaataacg ctgatagtgc tagtgtagat cgctactaga gattaaagag gagaaatact
      841 agatggccgt cactgattta agtttgacca attcttccct gatgcctacg ttgaacccga
      901 tgattcaaca gttggccctg gcgatcgccg ctagttggca aagtttaccc ctcaagccct
      961 atcaattgcc ggaggatttg ggctacgtag aaggccgcct ggaaggggaa aagttagtga
     1021 ttgaaaatcg gtgctaccaa acgccccagt ttcgcaaaat gcatttggag ttggccaagg
     1081 tgggcaaagg gttggatatt ctccactgtg taatgtttcc tgagccttta tacggtctac
     1141 ctttgtttgg ctgtgacatt gtggccggcc ccggtggagt aagtgcggct attgcggatc
     1201 tatcccccac ccaaagcgat cgccaattgc ccgcagcgta ccaaaaatca ttggcagagc
     1261 taggccagcc agaatttgag caacaacggg aattgccccc ctggggagaa atattttctg
     1321 aatattgttt attcatccgt cccagcaatg tcactgaaga agaaagattt gtacaaaggg
     1381 tagtggactt tttgcaaatt cattgtcacc aatccatcgt tgccgaaccc ttgtctgaag
     1441 ctcaaacttt ggagcaccgt caggggcaaa ttcattactg ccaacaacaa cagaaaaatg
     1501 ataaaacccg tcgggtactg gaaaaagctt ttggggaagc ttgggcggaa cggtatatga
     1561 gccaagtctt atttgatgtt atccaataat aactctgata gtgctagtgt agatctctac
     1621 tagagaaaga ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg
     1681 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
     1741 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
     1801 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
     1861 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
     1921 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
     1981 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
     2041 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
     2101 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     2161 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
     2221 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
     2281 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
     2341 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2401 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2461 ttata
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