LOCUS       BBa_K1132019            1080 bp    DNA     linear       08-May-2024
DEFINITION  term-FixK2 promoter-rbs-mRFP1-term
ACCESSION   BBa_K1132019
VERSION     BBa_K1132019.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=B1010
     stem_loop       10..31
                     /label=modified thr terminator
     polyA_site      7..9
                     /label=PolyA
     polyA_site      32..34
                     /label=PolyA
     terminator      1..40
                     /label=BBa_B1010
     promoter        49..298
                     /label=FixK2 promoter
     promoter        49..298
                     /label=BBa_K592006
     RBS             307..321
                     /label=RBS-1\Strong
     RBS             314..318
                     /label=RBS
     misc_feature    307..321
                     /label=BBa_B0030
     RBS             307..321
                     /label=BBa_B0030
     CDS             328..1002
                     /label=mrfp1
     misc_feature    1009..1033
                     /label=Help:Barcodes
     CDS             328..1033
                     /label=BBa_E1010
     misc_feature    1042..1080
                     /label=B1006
     stem_loop       1051..1072
                     /label=modified thr terminator
     polyA_site      1073..1080
                     /label=PolyA
     polyA_site      1042..1050
                     /label=PolyA
     terminator      1042..1080
                     /label=BBa_B1006
     misc_feature    307..1080
                     /label=BBa_K081014
ORIGIN
        1 cgccgcaaac cccgcccctg acagggcggg gtttcgccgc tactagaggc cggagccgat
       61 tatccgcacc cgtccttggt cttggacaca ctcgctcgcg atccgaagcc gcctttcaaa
      121 gatactccgc agtgaaacgc atcttttaag cgcgacttgt cgccttcgcg cagcagaaca
      181 ctcgtatggc actcaatcca tgatcgcttc gttcgctccg acgcgcgttg cggggccccc
      241 ctcgaccgga tgacaacaca ttgcgtcacc tcaactgctg cgcgcgcact gcgtcacata
      301 ctagagatta aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc
      361 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
      421 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
      481 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
      541 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
      601 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
      661 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
      721 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac
      781 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt
      841 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt
      901 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
      961 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
     1021 tagtgtagat cgctactaga gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt
//