LOCUS       BBa_K1132020            2884 bp    DNA     linear       03-May-2024
DEFINITION  rbs-YF1-rbs-FixJ-term-pFixK2-mRFP1
ACCESSION   BBa_K1132020
VERSION     BBa_K1132020.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1149
                     /label=YF1
     CDS             19..1149
                     /label=BBa_K592004
     misc_feature    1162..1165
                     /label=conserved
     RBS             1158..1169
                     /label=BBa_B0034
     CDS             1176..1796
                     /label=FixJ
     CDS             1176..1796
                     /label=BBa_K592005
     misc_feature    1..1796
                     /label=BBa_K592016
     misc_feature    1805..1844
                     /label=B1010
     stem_loop       1814..1835
                     /label=modified thr terminator
     polyA_site      1811..1813
                     /label=PolyA
     polyA_site      1836..1838
                     /label=PolyA
     terminator      1805..1844
                     /label=BBa_B1010
     promoter        1853..2102
                     /label=FixK2 promoter
     promoter        1853..2102
                     /label=BBa_K592006
     RBS             2111..2125
                     /label=RBS-1\Strong
     RBS             2118..2122
                     /label=RBS
     misc_feature    2111..2125
                     /label=BBa_B0030
     RBS             2111..2125
                     /label=BBa_B0030
     CDS             2132..2806
                     /label=mrfp1
     misc_feature    2813..2837
                     /label=Help:Barcodes
     CDS             2132..2837
                     /label=BBa_E1010
     misc_feature    2846..2884
                     /label=B1006
     stem_loop       2855..2876
                     /label=modified thr terminator
     polyA_site      2877..2884
                     /label=PolyA
     polyA_site      2846..2854
                     /label=PolyA
     terminator      2846..2884
                     /label=BBa_B1006
     misc_feature    2111..2884
                     /label=BBa_K081014
ORIGIN
        1 aaagaggaga aatactagat ggctagtttt caatcatttg ggataccagg acagctggaa
       61 gtcatcaaaa aagcacttga tcacgtgcga gtcggtgtgg taattacaga tcccgcactt
      121 gaagataatc ctattgtcta cgtaaatcaa ggctttgttc aaatgaccgg ctacgagacc
      181 gaggaaattt taggaaagaa ctgtcgcttc ttacagggga aacacacaga tccggcagaa
      241 gtggacaaca tcagaaccgc tttacaaaat aaagaaccgg tcaccgttca gatccaaaac
      301 tacaaaaaag acggaacgat gttctggaat gaattaaata ttgatccaat ggaaatagag
      361 gataaaacgt attttgtcgg cattcagaat gatatcaccg agcaccagca gacccaggcg
      421 cgtctccagg aactgcaatc cgagctcgtc cacgtctcca ggctgagcgc catgggcgaa
      481 atggcgtccg cgctcgcgca cgagctcaac cagccgctgg cggcgatcag caactacatg
      541 aagggctcgc ggcggctgct tgccggcagc agtgatccga acacaccgaa ggtcgaaagc
      601 gccctggacc gcgccgccga gcaggcgctg cgcgccggcc agatcatccg gcgcctgcgc
      661 gacttcgttg cccgcggcga atcggagaag cgggtcgaga gtctctccaa gctgatcgag
      721 gaggccggcg cgctcgggct tgccggcgcg cgcgagcaga acgtgcagct ccgcttcagt
      781 ctcgatccgg gcgccgatct cgttctcgcc gaccgggtgc agatccagca ggtcctggtc
      841 aacctgttcc gcaacgcgct ggaagcgatg gctcagtcgc agcgacgcga gctcgtcgtc
      901 accaacaccc ccgccgccga cgacatgatc gaggtcgaag tgtccgacac cggcagcggt
      961 ttccaggacg acgtcattcc gaacctgttt cagactttct tcaccaccaa ggacaccggc
     1021 atgggcgtgg gactgtccat cagccgctcg atcatcgaag ctcacggcgg gcgcatgtgg
     1081 gccgagagca acgcatcggg cggggcgacc ttccgcttca ccctcccggc agccgacgag
     1141 aattaataat actagagaaa gaggagaaat actagatgac gaccaaggga catatctacg
     1201 tcatcgacga cgacgcggcg atgcgggatt cgctgaattt cctgctggat tctgccggct
     1261 tcggcgtcac gctgtttgac gacgcgcaag cctttctcga cgccctgccg ggtctctcct
     1321 tcggctgcgt cgtctccgac gtgcgcatgc cgggccttga cggcatcgag ctgttgaagc
     1381 ggatgaaggc gcagcaaagc ccctttccga tcctcatcat gaccggtcac ggcgacgtgc
     1441 cgctcgcggt cgaggcgatg aagttagggg cggtcgactt tctggaaaag cctttcgagg
     1501 acgaccgcct caccgccatg atcgaatcgg cgatccgcca ggccgagccg gccgccaaga
     1561 gcgaggccgt cgcgcaggat atcgccgccc gcgtcgcctc gttgagcccc agggagcgcc
     1621 aggtcatgga agggctgatc gccggccttt ccaacaagct gatcgcccgc gagtacgaca
     1681 tcagcccgcg caccatcgag gtgtatcggg ccaacgtcat gaccaagatg caggccaaca
     1741 gcctttcgga gctggttcgc ctcgcgatgc gcgccggcat gctcaacgat taataatact
     1801 agagcgccgc aaaccccgcc cctgacaggg cggggtttcg ccgctactag aggccggagc
     1861 cgattatccg cacccgtcct tggtcttgga cacactcgct cgcgatccga agccgccttt
     1921 caaagatact ccgcagtgaa acgcatcttt taagcgcgac ttgtcgcctt cgcgcagcag
     1981 aacactcgta tggcactcaa tccatgatcg cttcgttcgc tccgacgcgc gttgcggggc
     2041 ccccctcgac cggatgacaa cacattgcgt cacctcaact gctgcgcgcg cactgcgtca
     2101 catactagag attaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga
     2161 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
     2221 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
     2281 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
     2341 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
     2401 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
     2461 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
     2521 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
     2581 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
     2641 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
     2701 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
     2761 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata
     2821 gtgctagtgt agatcgctac tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt
     2881 tttt
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