LOCUS       BBa_K1135002            1017 bp    DNA     linear       12-May-2024
DEFINITION  PcstA promoter-RBS-GFP-Terminator
ACCESSION   BBa_K1135002
VERSION     BBa_K1135002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     RBS             140..154
                     /label=RBS-1\Strong
     RBS             147..151
                     /label=RBS
     misc_feature    140..154
                     /label=BBa_B0030
     RBS             140..154
                     /label=BBa_B0030
     CDS             161..880
                     /label=GFP protein
     CDS             161..880
                     /label=BBa_E0040
     stem_loop       900..943
                     /label=stem_loop
     misc_feature    889..968
                     /label=BBa_B0010
     terminator      889..968
                     /label=BBa_B0010
     stem_loop       984..1003
                     /label=T7 TE
     misc_feature    1010..1010
                     /label=stop
     polyA_site      1004..1017
                     /label=polya
     misc_feature    977..1017
                     /label=BBa_B0012
     terminator      977..1017
                     /label=BBa_B0012
     misc_feature    140..1017
                     /label=BBa_E0840
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga ttaaagagga gaaatactag atgcgtaaag gagaagaact
      181 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      241 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      301 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      361 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      421 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      481 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      541 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      601 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      661 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      721 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      781 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      841 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
      901 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      961 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//