LOCUS       BBa_K1135003            1003 bp    DNA     linear       19-May-2024
DEFINITION  PcstA promoter-RBS-RFP-Terminator
ACCESSION   BBa_K1135003
VERSION     BBa_K1135003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     RBS             140..154
                     /label=RBS-1\Strong
     RBS             147..151
                     /label=RBS
     misc_feature    140..154
                     /label=BBa_B0030
     RBS             140..154
                     /label=BBa_B0030
     CDS             161..835
                     /label=mrfp1
     misc_feature    842..866
                     /label=Help:Barcodes
     CDS             161..866
                     /label=BBa_E1010
     stem_loop       886..929
                     /label=stem_loop
     misc_feature    875..954
                     /label=BBa_B0010
     terminator      875..954
                     /label=BBa_B0010
     stem_loop       970..989
                     /label=T7 TE
     misc_feature    996..996
                     /label=stop
     polyA_site      990..1003
                     /label=polya
     misc_feature    963..1003
                     /label=BBa_B0012
     terminator      963..1003
                     /label=BBa_B0012
     terminator      875..1003
                     /label=BBa_B0015
     misc_feature    140..1003
                     /label=BBa_K516030
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga ttaaagagga gaaatactag atggcttcct ccgaagacgt
      181 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      241 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      301 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      361 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      421 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      481 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      541 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      601 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      661 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      721 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      781 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata
      841 acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct
      901 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      961 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//