BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1136001 1 BBa_K1136001 Lyz 103 Endolysin from Erwinia amylovora bacteriophage 2013-09-15T11:00:00Z 2015-05-08T01:09:21Z This part comes from the genomic sequence of ERA103, a bacteriphage that targets Erwinia amylovora. This part codes for the Lyz103 endolysin protein from the bacteriophage ERA103, which targets Erwinia amylovora. This SAR endolysin is responsible for the lysis of the peptidoglycan layer of E. amylovora, a gram-negative bacterial pathogen which cause Fireblight in pear and apple trees. This part can be used to express Lyz103 for future projects related to cell lysis and bacterial disease. false false _1448_ 0 13007 9 In stock false The gene was synthesized by GenScript to optimize codons and avoid illegal cut sites. false Simin Jiang BBa_K1136004 1 BBa_K1136004 An L-Arabinose controlled endolysin from bacteriophage ERA103 2013-09-16T11:00:00Z 2015-05-08T01:09:21Z The endolysin gene is from bacteriophage ERA103. An E. coli promoter and terminator were used. L-arabinose induced Lyz103 is a functional construct which contains the gene that encodes for the endolysin protein from the bacteriophage ERA103 (BBa_K1136001). The construct is under the control of an L-arabinose promoter (BBa_K206000) and the rrnb terminator (BBa_B0010). This part can be expressed in E. coli using 20% L-Arabinose. false false _1448_ 0 13007 9 It's complicated false The endolysin gene was synthesized by GenScript to optimize codons and avoid illegal sites. The promotor and terminator were chosen for controllable expression, because the endolysin may be toxic in high copy number. false Simin Jiang component2351735 1 BBa_K206000 component2351736 1 BBa_K1136001 component2351737 1 BBa_B0010 annotation2351735 1 BBa_K206000 range2351735 1 1 130 annotation2351736 1 BBa_K1136001 range2351736 1 137 850 annotation2351737 1 BBa_B0010 range2351737 1 859 938 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 annotation2049254 1 AraI2 range2049254 1 61 78 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1136004_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgtttcgcctgatcctgacgctgctggtcgtggcagtgtcgttcaatacgcacgcctcttaccaatcgtcctcaaccaagcaacaaaaggaatttaaactgattgaacagcgtttcgaaaaagtccgcccgctgatcgtgaaggttgccaaagaacaaggcgttcatgcaggtaccctgaccacgaccatttatcgtgaatcgaagtttgatgtcaacgtgggcaaaaataaaaagagctctgcccacagcctggttcagatgacgatgccgaccaagcgctatgtcctgaaactgtacggcaaccaactgggtattccgaagaatgctgatctgaacaatccgaaatatgcggtgatgctggcggcggcatactttaaccatatcgaagaccacctgacgaaacagctgaagcgtaaaccgaccaatgccgaagtggcactgggttatcgcttcggcgaaggtgctgcggttgcgatgatcaaaaagaaaagttcctcaggcaaacgttggatggccagctttcgcaaagatgccgcattctacggtgcaaaggtcacgccgccgaaagtgattgaaacccatcagctggcttttgcggaaaagaaaccggctgttgacgtcacggaactgcaatctatctggaacaatctgtacaaaccggctgtgaacaccctggttgcgttcaacggcaatctgaaaggtgcatccctgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1136001_sequence 1 atgtttcgcctgatcctgacgctgctggtcgtggcagtgtcgttcaatacgcacgcctcttaccaatcgtcctcaaccaagcaacaaaaggaatttaaactgattgaacagcgtttcgaaaaagtccgcccgctgatcgtgaaggttgccaaagaacaaggcgttcatgcaggtaccctgaccacgaccatttatcgtgaatcgaagtttgatgtcaacgtgggcaaaaataaaaagagctctgcccacagcctggttcagatgacgatgccgaccaagcgctatgtcctgaaactgtacggcaaccaactgggtattccgaagaatgctgatctgaacaatccgaaatatgcggtgatgctggcggcggcatactttaaccatatcgaagaccacctgacgaaacagctgaagcgtaaaccgaccaatgccgaagtggcactgggttatcgcttcggcgaaggtgctgcggttgcgatgatcaaaaagaaaagttcctcaggcaaacgttggatggccagctttcgcaaagatgccgcattctacggtgcaaaggtcacgccgccgaaagtgattgaaacccatcagctggcttttgcggaaaagaaaccggctgttgacgtcacggaactgcaatctatctggaacaatctgtacaaaccggctgtgaacaccctggttgcgttcaacggcaatctgaaaggtgcatccctg BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z