BBa_K1136002
1
BBa_K1136002
KZ144 Endolysin from Pseudomonas aeruginosa bacteriophage
2013-09-15T11:00:00Z
2015-05-08T01:09:21Z
This part comes from genomic DNA sequence of Pseudomonas aeruginosa bacteriophage, ΦKZ.
This part is the sequence for the protein made from a bacteriophage that targets Pseudomonas aeruginosa and is responsible for lysing the bacteria. This protein is one of many kinds of endolysins. Endolysins typically have a high specificity and bacteria have not generally shown to become resistant to these proteins. There have been studies on how to vary the activity and specificity of this endolysin in particular, making it lyse other gram negative pathogens. This can be used in future projects that require the lysing of bacterial cells.
false
false
_1448_
0
18533
9
It's complicated
false
The gene was synthesized by Genescript to optimize codon and avoid EcoR1, PST, XBA, and SPE cut sites.
false
Christian Allen Sy
BBa_K1136005
1
BBa_K1136005
An L-Arabinose controlled endolysin from bacteriophage ΦKZ
2013-09-15T11:00:00Z
2015-05-08T01:09:21Z
The endolysin gene is from bacteriophage ΦKZ. An E. coli promoter and terminator were used.
L-arabinose induced KZ144 endolysin is a functional construct which contains the gene that encodes for the endolysin protein from the bacteriophage ΦKZ (BBa_K1136002) which targets Pseudomonas aeruginosa. The construct is under the control of an L-arabinose promoter (BBa_K206000) and the rrnb terminator (BBa_B0010). This part can be expressed in E. coli using 20% L-Arabinose.
false
false
_1448_
0
13007
9
It's complicated
false
The endolysin gene was synthesized by GenScript to optimize codons and avoid illegal sites. The promotor and terminator were chosen for controllable expression, because the endolysin may be toxic in high copy number.
false
Simin Jiang
component2351742
1
BBa_K206000
component2351743
1
BBa_K1136002
component2351744
1
BBa_B0010
annotation2351743
1
BBa_K1136002
range2351743
1
137
916
annotation2351742
1
BBa_K206000
range2351742
1
1
130
annotation2351744
1
BBa_B0010
range2351744
1
925
1004
BBa_K206000
1
pBAD
pBAD strong
2009-10-13T11:00:00Z
2015-05-08T01:11:23Z
The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
Released HQ 2013
pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
false
false
_307_
0
4172
9
In stock
true
There were no special design considerations.
false
Amelia Hardjasa
annotation2049254
1
AraI2
range2049254
1
61
78
annotation2049252
1
promoter
range2049252
1
1
131
annotation2049253
1
AraI1
range2049253
1
40
57
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1136002_sequence
1
atgaaagtgctgcgtaaaggcgaccgtggtgatgaagtgtgtcaactgcaaaccctgctgaacctgtgtggctatgatgtgggcaaaccggatggcatttttggtaacaataccttcaaccaggtggttaaatttcaaaaggacaattgcctggatagcgacggcatcgtgggtaaaaacacgtgggcagaactgttcagtaaatattccccgccgattccgtacaagaccatcccgatgccgacggcgaataaaagtcgtgcggcggcaaccccggtcatgaacgcagtggaaaatgcaaccggcgttcgctcacagctgctgctgacgtttgcctcaattgaatcggcattcgattatgaaatcaaagctaagaccagctctgccacgggctggtttcaattcctgaccggcacgtggaaaaccatgattgaaaactatggcatgaagtacggtgttctgaccgatccgacgggcgcactgcgtaaagacccgcgcatctccgctctgatgggtgcggaactgattaaagaaaacatgaatatcctgcgtccggtcctgaagcgcgaaccgaccgatacggacctgtatctggcgcatttctttggtccgggtgctgcacgtcgctttctgaccacgggccagaatgaactggccgcaacccactttccgaaagaagctcaagcgaacccgagcattttctacaataaggatggttctccgaagacgattcaagaagtttacaacctgatggatggcaaagtggctgcacaccgcaag
BBa_K206000_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_K1136005_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgaaagtgctgcgtaaaggcgaccgtggtgatgaagtgtgtcaactgcaaaccctgctgaacctgtgtggctatgatgtgggcaaaccggatggcatttttggtaacaataccttcaaccaggtggttaaatttcaaaaggacaattgcctggatagcgacggcatcgtgggtaaaaacacgtgggcagaactgttcagtaaatattccccgccgattccgtacaagaccatcccgatgccgacggcgaataaaagtcgtgcggcggcaaccccggtcatgaacgcagtggaaaatgcaaccggcgttcgctcacagctgctgctgacgtttgcctcaattgaatcggcattcgattatgaaatcaaagctaagaccagctctgccacgggctggtttcaattcctgaccggcacgtggaaaaccatgattgaaaactatggcatgaagtacggtgttctgaccgatccgacgggcgcactgcgtaaagacccgcgcatctccgctctgatgggtgcggaactgattaaagaaaacatgaatatcctgcgtccggtcctgaagcgcgaaccgaccgatacggacctgtatctggcgcatttctttggtccgggtgctgcacgtcgctttctgaccacgggccagaatgaactggccgcaacccactttccgaaagaagctcaagcgaacccgagcattttctacaataaggatggttctccgaagacgattcaagaagtttacaacctgatggatggcaaagtggctgcacaccgcaagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z