LOCUS       BBa_K1139152            1966 bp    DNA     linear       21-May-2024
DEFINITION  Prm/lac V1-GFP-PlacIq-c1
ACCESSION   BBa_K1139152
VERSION     BBa_K1139152.1  
FEATURES             Location/Qualifiers
     misc_feature    3..18
                     /label=OR1
     misc_feature    27..42
                     /label=OR2
     promoter        42..47
                     /label=-35
     misc_feature    48..64
                     /label=lacO
     promoter        65..69
                     /label=-10
     misc_feature    79..95
                     /label=lacO
     promoter        1..97
                     /label=BBa_K1139153
     RBS             106..120
                     /label=RBS-1\Strong
     RBS             113..117
                     /label=RBS
     misc_feature    106..120
                     /label=BBa_B0030
     RBS             106..120
                     /label=BBa_B0030
     CDS             127..846
                     /label=GFP protein
     CDS             127..846
                     /label=BBa_E0040
     stem_loop       866..909
                     /label=stem_loop
     misc_feature    855..934
                     /label=BBa_B0010
     terminator      855..934
                     /label=BBa_B0010
     stem_loop       950..969
                     /label=T7 TE
     misc_feature    976..976
                     /label=stop
     polyA_site      970..983
                     /label=polya
     misc_feature    943..983
                     /label=BBa_B0012
     terminator      943..983
                     /label=BBa_B0012
     misc_feature    992..1028
                     /label=P(Lac) IQ
     promoter        994..999
                     /label=-35
     promoter        1017..1022
                     /label=-10
     promoter        992..1028
                     /label=BBa_I14032
     misc_feature    1041..1044
                     /label=conserved
     RBS             1037..1048
                     /label=BBa_B0034
     CDS             1058..1765
                     /label=cI lambda
     misc_feature    1766..1798
                     /label=LVA
     misc_feature    1805..1829
                     /label=Help:Barcodes
     CDS             1055..1804
                     /label=BBa_C0051
     stem_loop       1849..1892
                     /label=stem_loop
     misc_feature    1838..1917
                     /label=BBa_B0010
     terminator      1838..1917
                     /label=BBa_B0010
     stem_loop       1933..1952
                     /label=T7 TE
     misc_feature    1959..1959
                     /label=stop
     polyA_site      1953..1966
                     /label=polya
     misc_feature    1926..1966
                     /label=BBa_B0012
     terminator      1926..1966
                     /label=BBa_B0012
     misc_feature    1037..1966
                     /label=BBa_P0451
ORIGIN
        1 cctatcaccg ccagaggtaa aatagtcaac acgcacggtg ttagatattg tgagcggata
       61 acaatagatt tgtggcaatt gtgagcggat aacaatttac tagagattaa agaggagaaa
      121 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      181 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      241 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      301 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      361 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      421 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      481 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      541 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      601 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      661 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      721 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      781 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      841 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      901 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      961 gggtgggcct ttctgcgttt atatactaga gtggtgcaaa acctttcgcg gtatggcatg
     1021 atagcgccta ctagagaaag aggagaaata ctagatgagc acaaaaaaga aaccattaac
     1081 acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga
     1141 acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg
     1201 tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc ttgcaaaaat
     1261 tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta
     1321 tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca
     1381 tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag
     1441 atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa
     1501 ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt taattctcgt
     1561 tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg ggggtgatga
     1621 gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac cactaaaccc
     1681 acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag ttatcgctag
     1741 tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt tagtagctta
     1801 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
     1861 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1921 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//