LOCUS       BBa_K1139154            1967 bp    DNA     linear       22-May-2024
DEFINITION  Prm/lac V2-GFP-PlacIq-c1
ACCESSION   BBa_K1139154
VERSION     BBa_K1139154.1  
FEATURES             Location/Qualifiers
     misc_feature    3..18
                     /label=OR1
     misc_feature    27..42
                     /label=OR2
     promoter        42..47
                     /label=-35
     misc_feature    48..64
                     /label=lacO
     promoter        65..69
                     /label=-10
     misc_feature    80..96
                     /label=lacO
     promoter        1..98
                     /label=BBa_K1139155
     RBS             107..121
                     /label=RBS-1\Strong
     RBS             114..118
                     /label=RBS
     misc_feature    107..121
                     /label=BBa_B0030
     RBS             107..121
                     /label=BBa_B0030
     CDS             128..847
                     /label=GFP protein
     CDS             128..847
                     /label=BBa_E0040
     stem_loop       867..910
                     /label=stem_loop
     misc_feature    856..935
                     /label=BBa_B0010
     terminator      856..935
                     /label=BBa_B0010
     stem_loop       951..970
                     /label=T7 TE
     misc_feature    977..977
                     /label=stop
     polyA_site      971..984
                     /label=polya
     misc_feature    944..984
                     /label=BBa_B0012
     terminator      944..984
                     /label=BBa_B0012
     misc_feature    993..1029
                     /label=P(Lac) IQ
     promoter        995..1000
                     /label=-35
     promoter        1018..1023
                     /label=-10
     promoter        993..1029
                     /label=BBa_I14032
     misc_feature    1042..1045
                     /label=conserved
     RBS             1038..1049
                     /label=BBa_B0034
     CDS             1059..1766
                     /label=cI lambda
     misc_feature    1767..1799
                     /label=LVA
     misc_feature    1806..1830
                     /label=Help:Barcodes
     CDS             1056..1805
                     /label=BBa_C0051
     stem_loop       1850..1893
                     /label=stem_loop
     misc_feature    1839..1918
                     /label=BBa_B0010
     terminator      1839..1918
                     /label=BBa_B0010
     stem_loop       1934..1953
                     /label=T7 TE
     misc_feature    1960..1960
                     /label=stop
     polyA_site      1954..1967
                     /label=polya
     misc_feature    1927..1967
                     /label=BBa_B0012
     terminator      1927..1967
                     /label=BBa_B0012
     misc_feature    1038..1967
                     /label=BBa_P0451
ORIGIN
        1 cctatcaccg ccagaggtaa aatagtcaac acgcacggtg ttagatattg tgagcggata
       61 acaatagatt tgtggcgaat tgtgagcgga taacaattta ctagagatta aagaggagaa
      121 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      181 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      241 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      301 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      361 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      421 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      481 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      541 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      601 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      661 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      721 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
      781 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
      841 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      961 cgggtgggcc tttctgcgtt tatatactag agtggtgcaa aacctttcgc ggtatggcat
     1021 gatagcgcct actagagaaa gaggagaaat actagatgag cacaaaaaag aaaccattaa
     1081 cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg
     1141 aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag tcaggcgttg
     1201 gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg cttgcaaaaa
     1261 ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc tacgagatgt
     1321 atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct gttttttctc
     1381 atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt gatgcggaga
     1441 gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag gttgaaggta
     1501 attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg ttaattctcg
     1561 ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt gggggtgatg
     1621 agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa ccactaaacc
     1681 cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta
     1741 gtcagtggcc tgaagagacg tttggcgctg caaacgacga aaactacgct ttagtagctt
     1801 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
     1861 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1921 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//