LOCUS       BBa_K1139156            1968 bp    DNA     linear       21-May-2024
DEFINITION  Prm/lac V3-GFP-PlacIq-c1
ACCESSION   BBa_K1139156
VERSION     BBa_K1139156.1  
FEATURES             Location/Qualifiers
     misc_feature    3..18
                     /label=OR1
     misc_feature    27..42
                     /label=OR2
     promoter        42..47
                     /label=-35
     misc_feature    48..64
                     /label=lacO
     promoter        65..69
                     /label=-10
     misc_feature    81..97
                     /label=lacO
     promoter        1..99
                     /label=BBa_K1139157
     RBS             108..122
                     /label=RBS-1\Strong
     RBS             115..119
                     /label=RBS
     misc_feature    108..122
                     /label=BBa_B0030
     RBS             108..122
                     /label=BBa_B0030
     CDS             129..848
                     /label=GFP protein
     CDS             129..848
                     /label=BBa_E0040
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    994..1030
                     /label=P(Lac) IQ
     promoter        996..1001
                     /label=-35
     promoter        1019..1024
                     /label=-10
     promoter        994..1030
                     /label=BBa_I14032
     misc_feature    1043..1046
                     /label=conserved
     RBS             1039..1050
                     /label=BBa_B0034
     CDS             1060..1767
                     /label=cI lambda
     misc_feature    1768..1800
                     /label=LVA
     misc_feature    1807..1831
                     /label=Help:Barcodes
     CDS             1057..1806
                     /label=BBa_C0051
     stem_loop       1851..1894
                     /label=stem_loop
     misc_feature    1840..1919
                     /label=BBa_B0010
     terminator      1840..1919
                     /label=BBa_B0010
     stem_loop       1935..1954
                     /label=T7 TE
     misc_feature    1961..1961
                     /label=stop
     polyA_site      1955..1968
                     /label=polya
     misc_feature    1928..1968
                     /label=BBa_B0012
     terminator      1928..1968
                     /label=BBa_B0012
     misc_feature    1039..1968
                     /label=BBa_P0451
ORIGIN
        1 cctatcaccg ccagaggtaa aatagtcaac acgcacggtg ttagatattg tgagcggata
       61 acaatagatt tgtggcgtaa ttgtgagcgg ataacaattt actagagatt aaagaggaga
      121 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      181 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      241 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      301 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
      361 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      421 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      481 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      541 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
      601 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      661 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      721 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
      781 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
      841 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtggtgca aaacctttcg cggtatggca
     1021 tgatagcgcc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta
     1081 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat
     1141 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt
     1201 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa
     1261 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg
     1321 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct
     1381 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag
     1441 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt
     1501 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc
     1561 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat
     1621 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac
     1681 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct
     1741 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct
     1801 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     1861 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1921 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//