LOCUS       BBa_K1149002            2703 bp    DNA     linear       23-May-2024
DEFINITION  pelB-EstCS2-his expression (GFP reporter)
ACCESSION   BBa_K1149002
VERSION     BBa_K1149002.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..222
                     /label=PelB
     misc_feature    157..222
                     /label=BBa_J32015
     misc_feature    1932..1959
                     /label=6his3stop
     CDS             223..1959
                     /label=BBa_K1149030
     RBS             1960..1983
                     /label=rbs
     CDS             1984..2703
                     /label=sfGFP
     misc_feature    1960..2703
                     /label=BBa_K515005
     misc_feature    157..2703
                     /label=BBa_S05214
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aatacctgct gccgaccgct
      181 gctgctggtc tgctgctcct cgctgcccag ccggcgatgg ccatgatgcg agccgagttg
      241 catcgctctc cccgacaaca ccgtctcggc aaacaacgac aacatgagga ggatgaccca
      301 gtgcgaacca aattcggcgc ctggaagccg ctgaccgcgt tggcatcggc gatcctgttg
      361 gccgcctctg tggctggctg tggctcaagt tcgaaatcgg acgacaggga acccccaccg
      421 gctccgagaa tgggcaacta tcagggaacc gacgaaggca gcatgcttgc cttccgcggc
      481 attcgctacg ccaagccgcc cgtaggcgaa ctgcggttcg cgccacccca gccggtcgag
      541 aagccaacgg ggattgtggt cgccgataaa ttcggcagcg cctgtcccca gtaccaaagc
      601 gccttcggcc cggaaagcct cgaagaggac tgccttttcc tcaacgtgta cgtccccaag
      661 gctggcgagg gccccttccc ggtcatggtc tggatccatg gcggcgcatt cgttgctggt
      721 tcaggcggtc cagaatatga cccctcacgc ttggtagccc agggtgtggt ggtcgtcgcc
      781 atcaactatc gcctgggccc gctcggcttt ctcgcccatc cggatttaac caacgaacaa
      841 ggcggcggtt cgggcaacta tggcctgatg gatcagcagt tagcgctgcg ctgggtccag
      901 gacaatatct caacctttgg cggcgatcca aacaacgtca ctatctttgg tgaatcagcc
      961 ggcggcctta gcgtgctgtc gcatgtcgta tcgcctaagg cacagggtct cttccagcgc
     1021 gctatcgtcc aaagcggctc gtacgatgca atcagccgca ccttggctga ggccgagcaa
     1081 gcaggtcagg atttcgtagc tgccgtgggt tgcgactcgg caggggatat tccgcaatgc
     1141 ctgcgtgaac tggatgtcaa gaccattctc gacaatggcg atgcaatcgt agccggcctc
     1201 tcgctgacgc cccatttaca ccccgacctg ctgccgaaat caattaacca ggctttgcaa
     1261 gaaggcaaag ttaacgttaa ttctattctc ctcggtagca atcgcgacga gttcacgtta
     1321 ttcatagccc ttgaagaact caatggaact cccccaatgg aggaagatga tgtcgaagat
     1381 caatggtcta aattttttga ccctttgact tccatagtaa gcacttacta tcctgctacc
     1441 gactttgaaa accccagccg tcgcttcgct gcaccgttgg gtgacagcgt tttcagctgt
     1501 accgccttgc ggcaagcaga acagctaggc agcaccatcc ccgtctatgt ctacgagttc
     1561 gctgaccgcg atgctcccag cattctgcct aaggtcagct tcgaccttgg cgctgcccat
     1621 gctttcgaaa tccagtatgt actaggcagc gagcaagccc tgcgggcacg cggagcgaat
     1681 gattcgcaaa ttgcgctttc caacgccatg gttcaatact ggactgcttt cgcccgtacc
     1741 ggcgatccca atcaagggga ggcggaagca atttggccgg agtttggaag caataacggc
     1801 aatattcgct ggctaaaccc cgccgaacct gagaaaacgg tgatcacgag cgctgagttc
     1861 gaggcagatc accgttgcga aatttgggat aaaataatat tccaagctct taaacaagat
     1921 ccccaaaaat cacaccatca ccatcaccat taatagtgaa aatcaaatta aggaggtaag
     1981 ataatgcgta aaggcgaaga actgttcacg ggcgtagttc cgattctggt cgagctggac
     2041 ggcgatgtga acggtcataa gtttagcgtt cgcggtgaag gtgagggcga cgcgaccaac
     2101 ggcaaactga ccctgaagtt catctgcacc accggtaaac tgccggtgcc ttggccgacc
     2161 ttggtgacga cgttgacgta tggcgtgcag tgttttgcgc gttatccgga ccacatgaaa
     2221 caacacgatt tcttcaaatc tgcgatgccg gagggttacg tccaggagcg taccatttcc
     2281 ttcaaggatg atggctacta caaaactcgc gcagaggtta agtttgaagg tgacacgctg
     2341 gtcaatcgta tcgaattgaa gggtatcgac tttaaagagg atggtaacat tctgggccat
     2401 aaactggagt ataacttcaa cagccataat gtttacatta cggcagacaa gcaaaagaac
     2461 ggcatcaagg ccaatttcaa gattcgccac aatgttgagg acggtagcgt ccaactggcc
     2521 gaccattacc agcagaacac cccaattggt gacggtccgg ttttgctgcc ggataatcac
     2581 tatctgagca cccaaagcgt gctgagcaaa gatccgaacg aaaaacgtga tcacatggtc
     2641 ctgctggaat ttgtgaccgc tgcgggcatc acccacggta tggacgagct gtataagtaa
     2701 tga
//