BBa_K1149002 1 BBa_K1149002 pelB-EstCS2-his expression (GFP reporter) 2013-09-12T11:00:00Z 2015-05-08T01:09:26Z false false _1461_ 0 17968 9 In stock true false Margarita Kopniczky component2343315 1 BBa_K206000 component2343317 1 BBa_B0034 component2343325 1 BBa_S05214 annotation2343325 1 BBa_S05214 range2343325 1 157 2703 annotation2343317 1 BBa_B0034 range2343317 1 139 150 annotation2343315 1 BBa_K206000 range2343315 1 1 130 BBa_K515005 1 BBa_K515005 superfolder GFP (sfGFP) 2011-09-06T11:00:00Z 2015-05-08T01:12:32Z - a translational unit of superfolded GFP false false _680_ 0 10179 9 Not in stock false - false Atipat Patharagulpong annotation2126852 1 sfGFP range2126852 1 25 744 annotation2126851 1 rbs range2126851 1 1 24 BBa_J32015 1 PelB PelB leader sequence; directs protein to E. coli periplasmic membrane 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Novagen Released HQ 2013 The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface. false true _50_ 0 495 50 In stock true . true Austen Heinz annotation1898182 1 PelB range1898182 1 1 66 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 annotation2049254 1 AraI2 range2049254 1 61 78 BBa_S05214 1 BBa_S05214 K1149022:K1149030 2013-09-12T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 17968 9 Not in stock false false Margarita Kopniczky component2343306 1 BBa_J32015 component2343308 1 BBa_K1149030 component2343311 1 BBa_K515005 annotation2343306 1 BBa_J32015 range2343306 1 1 66 annotation2343311 1 BBa_K515005 range2343311 1 1804 2547 annotation2343308 1 BBa_K1149030 range2343308 1 67 1803 BBa_K1149030 1 BBa_K1149030 ESTCS2-His 2013-09-12T11:00:00Z 2015-05-08T01:09:26Z PUR degradation enzyme with His tag. It is from [http://parts.igem.org/Part:BBa_K892012 BBa_K892012] previous biobrick. false false _1461_ 0 17968 9 Not in stock false 6x His tag added. false Margarita Kopniczky annotation2339841 1 6his3stop range2339841 1 1710 1737 BBa_K1149030_sequence 1 atgatgcgagccgagttgcatcgctctccccgacaacaccgtctcggcaaacaacgacaacatgaggaggatgacccagtgcgaaccaaattcggcgcctggaagccgctgaccgcgttggcatcggcgatcctgttggccgcctctgtggctggctgtggctcaagttcgaaatcggacgacagggaacccccaccggctccgagaatgggcaactatcagggaaccgacgaaggcagcatgcttgccttccgcggcattcgctacgccaagccgcccgtaggcgaactgcggttcgcgccaccccagccggtcgagaagccaacggggattgtggtcgccgataaattcggcagcgcctgtccccagtaccaaagcgccttcggcccggaaagcctcgaagaggactgccttttcctcaacgtgtacgtccccaaggctggcgagggccccttcccggtcatggtctggatccatggcggcgcattcgttgctggttcaggcggtccagaatatgacccctcacgcttggtagcccagggtgtggtggtcgtcgccatcaactatcgcctgggcccgctcggctttctcgcccatccggatttaaccaacgaacaaggcggcggttcgggcaactatggcctgatggatcagcagttagcgctgcgctgggtccaggacaatatctcaacctttggcggcgatccaaacaacgtcactatctttggtgaatcagccggcggccttagcgtgctgtcgcatgtcgtatcgcctaaggcacagggtctcttccagcgcgctatcgtccaaagcggctcgtacgatgcaatcagccgcaccttggctgaggccgagcaagcaggtcaggatttcgtagctgccgtgggttgcgactcggcaggggatattccgcaatgcctgcgtgaactggatgtcaagaccattctcgacaatggcgatgcaatcgtagccggcctctcgctgacgccccatttacaccccgacctgctgccgaaatcaattaaccaggctttgcaagaaggcaaagttaacgttaattctattctcctcggtagcaatcgcgacgagttcacgttattcatagcccttgaagaactcaatggaactcccccaatggaggaagatgatgtcgaagatcaatggtctaaattttttgaccctttgacttccatagtaagcacttactatcctgctaccgactttgaaaaccccagccgtcgcttcgctgcaccgttgggtgacagcgttttcagctgtaccgccttgcggcaagcagaacagctaggcagcaccatccccgtctatgtctacgagttcgctgaccgcgatgctcccagcattctgcctaaggtcagcttcgaccttggcgctgcccatgctttcgaaatccagtatgtactaggcagcgagcaagccctgcgggcacgcggagcgaatgattcgcaaattgcgctttccaacgccatggttcaatactggactgctttcgcccgtaccggcgatcccaatcaaggggaggcggaagcaatttggccggagtttggaagcaataacggcaatattcgctggctaaaccccgccgaacctgagaaaacggtgatcacgagcgctgagttcgaggcagatcaccgttgcgaaatttgggataaaataatattccaagctcttaaacaagatccccaaaaatcacaccatcaccatcaccattaatagtga BBa_K515005_sequence 1 aaatcaaattaaggaggtaagataatgcgtaaaggcgaagaactgttcacgggcgtagttccgattctggtcgagctggacggcgatgtgaacggtcataagtttagcgttcgcggtgaaggtgagggcgacgcgaccaacggcaaactgaccctgaagttcatctgcaccaccggtaaactgccggtgccttggccgaccttggtgacgacgttgacgtatggcgtgcagtgttttgcgcgttatccggaccacatgaaacaacacgatttcttcaaatctgcgatgccggagggttacgtccaggagcgtaccatttccttcaaggatgatggctactacaaaactcgcgcagaggttaagtttgaaggtgacacgctggtcaatcgtatcgaattgaagggtatcgactttaaagaggatggtaacattctgggccataaactggagtataacttcaacagccataatgtttacattacggcagacaagcaaaagaacggcatcaaggccaatttcaagattcgccacaatgttgaggacggtagcgtccaactggccgaccattaccagcagaacaccccaattggtgacggtccggttttgctgccggataatcactatctgagcacccaaagcgtgctgagcaaagatccgaacgaaaaacgtgatcacatggtcctgctggaatttgtgaccgctgcgggcatcacccacggtatggacgagctgtataagtaatga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1149002_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgatgcgagccgagttgcatcgctctccccgacaacaccgtctcggcaaacaacgacaacatgaggaggatgacccagtgcgaaccaaattcggcgcctggaagccgctgaccgcgttggcatcggcgatcctgttggccgcctctgtggctggctgtggctcaagttcgaaatcggacgacagggaacccccaccggctccgagaatgggcaactatcagggaaccgacgaaggcagcatgcttgccttccgcggcattcgctacgccaagccgcccgtaggcgaactgcggttcgcgccaccccagccggtcgagaagccaacggggattgtggtcgccgataaattcggcagcgcctgtccccagtaccaaagcgccttcggcccggaaagcctcgaagaggactgccttttcctcaacgtgtacgtccccaaggctggcgagggccccttcccggtcatggtctggatccatggcggcgcattcgttgctggttcaggcggtccagaatatgacccctcacgcttggtagcccagggtgtggtggtcgtcgccatcaactatcgcctgggcccgctcggctttctcgcccatccggatttaaccaacgaacaaggcggcggttcgggcaactatggcctgatggatcagcagttagcgctgcgctgggtccaggacaatatctcaacctttggcggcgatccaaacaacgtcactatctttggtgaatcagccggcggccttagcgtgctgtcgcatgtcgtatcgcctaaggcacagggtctcttccagcgcgctatcgtccaaagcggctcgtacgatgcaatcagccgcaccttggctgaggccgagcaagcaggtcaggatttcgtagctgccgtgggttgcgactcggcaggggatattccgcaatgcctgcgtgaactggatgtcaagaccattctcgacaatggcgatgcaatcgtagccggcctctcgctgacgccccatttacaccccgacctgctgccgaaatcaattaaccaggctttgcaagaaggcaaagttaacgttaattctattctcctcggtagcaatcgcgacgagttcacgttattcatagcccttgaagaactcaatggaactcccccaatggaggaagatgatgtcgaagatcaatggtctaaattttttgaccctttgacttccatagtaagcacttactatcctgctaccgactttgaaaaccccagccgtcgcttcgctgcaccgttgggtgacagcgttttcagctgtaccgccttgcggcaagcagaacagctaggcagcaccatccccgtctatgtctacgagttcgctgaccgcgatgctcccagcattctgcctaaggtcagcttcgaccttggcgctgcccatgctttcgaaatccagtatgtactaggcagcgagcaagccctgcgggcacgcggagcgaatgattcgcaaattgcgctttccaacgccatggttcaatactggactgctttcgcccgtaccggcgatcccaatcaaggggaggcggaagcaatttggccggagtttggaagcaataacggcaatattcgctggctaaaccccgccgaacctgagaaaacggtgatcacgagcgctgagttcgaggcagatcaccgttgcgaaatttgggataaaataatattccaagctcttaaacaagatccccaaaaatcacaccatcaccatcaccattaatagtgaaaatcaaattaaggaggtaagataatgcgtaaaggcgaagaactgttcacgggcgtagttccgattctggtcgagctggacggcgatgtgaacggtcataagtttagcgttcgcggtgaaggtgagggcgacgcgaccaacggcaaactgaccctgaagttcatctgcaccaccggtaaactgccggtgccttggccgaccttggtgacgacgttgacgtatggcgtgcagtgttttgcgcgttatccggaccacatgaaacaacacgatttcttcaaatctgcgatgccggagggttacgtccaggagcgtaccatttccttcaaggatgatggctactacaaaactcgcgcagaggttaagtttgaaggtgacacgctggtcaatcgtatcgaattgaagggtatcgactttaaagaggatggtaacattctgggccataaactggagtataacttcaacagccataatgtttacattacggcagacaagcaaaagaacggcatcaaggccaatttcaagattcgccacaatgttgaggacggtagcgtccaactggccgaccattaccagcagaacaccccaattggtgacggtccggttttgctgccggataatcactatctgagcacccaaagcgtgctgagcaaagatccgaacgaaaaacgtgatcacatggtcctgctggaatttgtgaccgctgcgggcatcacccacggtatggacgagctgtataagtaatga BBa_J32015_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc BBa_S05214_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgatgcgagccgagttgcatcgctctccccgacaacaccgtctcggcaaacaacgacaacatgaggaggatgacccagtgcgaaccaaattcggcgcctggaagccgctgaccgcgttggcatcggcgatcctgttggccgcctctgtggctggctgtggctcaagttcgaaatcggacgacagggaacccccaccggctccgagaatgggcaactatcagggaaccgacgaaggcagcatgcttgccttccgcggcattcgctacgccaagccgcccgtaggcgaactgcggttcgcgccaccccagccggtcgagaagccaacggggattgtggtcgccgataaattcggcagcgcctgtccccagtaccaaagcgccttcggcccggaaagcctcgaagaggactgccttttcctcaacgtgtacgtccccaaggctggcgagggccccttcccggtcatggtctggatccatggcggcgcattcgttgctggttcaggcggtccagaatatgacccctcacgcttggtagcccagggtgtggtggtcgtcgccatcaactatcgcctgggcccgctcggctttctcgcccatccggatttaaccaacgaacaaggcggcggttcgggcaactatggcctgatggatcagcagttagcgctgcgctgggtccaggacaatatctcaacctttggcggcgatccaaacaacgtcactatctttggtgaatcagccggcggccttagcgtgctgtcgcatgtcgtatcgcctaaggcacagggtctcttccagcgcgctatcgtccaaagcggctcgtacgatgcaatcagccgcaccttggctgaggccgagcaagcaggtcaggatttcgtagctgccgtgggttgcgactcggcaggggatattccgcaatgcctgcgtgaactggatgtcaagaccattctcgacaatggcgatgcaatcgtagccggcctctcgctgacgccccatttacaccccgacctgctgccgaaatcaattaaccaggctttgcaagaaggcaaagttaacgttaattctattctcctcggtagcaatcgcgacgagttcacgttattcatagcccttgaagaactcaatggaactcccccaatggaggaagatgatgtcgaagatcaatggtctaaattttttgaccctttgacttccatagtaagcacttactatcctgctaccgactttgaaaaccccagccgtcgcttcgctgcaccgttgggtgacagcgttttcagctgtaccgccttgcggcaagcagaacagctaggcagcaccatccccgtctatgtctacgagttcgctgaccgcgatgctcccagcattctgcctaaggtcagcttcgaccttggcgctgcccatgctttcgaaatccagtatgtactaggcagcgagcaagccctgcgggcacgcggagcgaatgattcgcaaattgcgctttccaacgccatggttcaatactggactgctttcgcccgtaccggcgatcccaatcaaggggaggcggaagcaatttggccggagtttggaagcaataacggcaatattcgctggctaaaccccgccgaacctgagaaaacggtgatcacgagcgctgagttcgaggcagatcaccgttgcgaaatttgggataaaataatattccaagctcttaaacaagatccccaaaaatcacaccatcaccatcaccattaatagtgaaaatcaaattaaggaggtaagataatgcgtaaaggcgaagaactgttcacgggcgtagttccgattctggtcgagctggacggcgatgtgaacggtcataagtttagcgttcgcggtgaaggtgagggcgacgcgaccaacggcaaactgaccctgaagttcatctgcaccaccggtaaactgccggtgccttggccgaccttggtgacgacgttgacgtatggcgtgcagtgttttgcgcgttatccggaccacatgaaacaacacgatttcttcaaatctgcgatgccggagggttacgtccaggagcgtaccatttccttcaaggatgatggctactacaaaactcgcgcagaggttaagtttgaaggtgacacgctggtcaatcgtatcgaattgaagggtatcgactttaaagaggatggtaacattctgggccataaactggagtataacttcaacagccataatgtttacattacggcagacaagcaaaagaacggcatcaaggccaatttcaagattcgccacaatgttgaggacggtagcgtccaactggccgaccattaccagcagaacaccccaattggtgacggtccggttttgctgccggataatcactatctgagcacccaaagcgtgctgagcaaagatccgaacgaaaaacgtgatcacatggtcctgctggaatttgtgaccgctgcgggcatcacccacggtatggacgagctgtataagtaatga BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z