LOCUS       BBa_K1149008            2103 bp    DNA     linear       18-May-2024
DEFINITION  Proteinase K (extracellular)
ACCESSION   BBa_K1149008
VERSION     BBa_K1149008.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..222
                     /label=BBa_K1149023
     misc_feature    1338..1359
                     /label=6his+stop
     CDS             223..1359
                     /label=BBa_K1149035
     RBS             1360..1383
                     /label=rbs
     CDS             1384..2103
                     /label=sfGFP
     misc_feature    1360..2103
                     /label=BBa_K515005
     misc_feature    157..2103
                     /label=BBa_S05217
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aatacctgct gccgaccgct
      181 gctgctggtc tgctgctcct cgctgcccag ccggcgatgg ccatggcacc ggccgttgaa
      241 cagcgttctg aagcagctcc tctgattgag gcacgtggtg aaatggtagc aaacaagtac
      301 atcgtgaagt tcaaggaggg ttctgctctg tctgctctgg atgctgctat ggaaaagatc
      361 tctggcaagc ctgatcacgt ctataagaac gtgttcagcg gtttcgcagc aactctggac
      421 gagaacatgg tccgtgtact gcgtgctcat ccagacgttg aatacatcga acaggacgct
      481 gtggttacta tcaacgcggc acagactaac gcaccttggg gtctggcacg tatttcttct
      541 acttccccgg gtacgtctac ttactactac gacgagtctg ccggtcaagg ttcttgcgtt
      601 tacgtgatcg atacgggcat cgaggcttct catcctgagt ttgaaggccg tgcacaaatg
      661 gtgaagacct actactactc ttcccgtgac ggtaatggtc acggtactca ttgcgcaggt
      721 actgttggta gccgtaccta cggtgttgct aagaaaacgc aactgttcgg cgttaaagtg
      781 ctggacgaca acggttctgg tcagtactcc accattatcg cgggtatgga tttcgtagcg
      841 agcgataaaa acaaccgcaa ctgcccgaaa ggtgttgtgg cttctctgtc tctgggtggt
      901 ggttactcct cttctgttaa cagcgcagct gcacgtctgc aatcttccgg tgtcatggtc
      961 gcagtagcag ctggtaacaa taacgctgat gcacgcaact actctcctgc tagcgagcct
     1021 tctgtttgca ccgtgggtgc atctgatcgt tatgatcgtc gtagctcctt cagcaactat
     1081 ggttccgtcc tggatatctt cggccctggt acttctatcc tgtctacctg gattggcggt
     1141 agcactcgtt ccatttccgg tacgagcatg gctactccac atgttgctgg tctggcagca
     1201 tacctgatga ccctgggtaa gaccactgct gcatccgctt gtcgttacat cgcggatact
     1261 gcgaacaaag gcgatctgtc taacatcccg ttcggcaccg ttaatctgct ggcatacaac
     1321 aactatcagg ctgtcgacca tcatcatcat catcattgaa aatcaaatta aggaggtaag
     1381 ataatgcgta aaggcgaaga actgttcacg ggcgtagttc cgattctggt cgagctggac
     1441 ggcgatgtga acggtcataa gtttagcgtt cgcggtgaag gtgagggcga cgcgaccaac
     1501 ggcaaactga ccctgaagtt catctgcacc accggtaaac tgccggtgcc ttggccgacc
     1561 ttggtgacga cgttgacgta tggcgtgcag tgttttgcgc gttatccgga ccacatgaaa
     1621 caacacgatt tcttcaaatc tgcgatgccg gagggttacg tccaggagcg taccatttcc
     1681 ttcaaggatg atggctacta caaaactcgc gcagaggtta agtttgaagg tgacacgctg
     1741 gtcaatcgta tcgaattgaa gggtatcgac tttaaagagg atggtaacat tctgggccat
     1801 aaactggagt ataacttcaa cagccataat gtttacatta cggcagacaa gcaaaagaac
     1861 ggcatcaagg ccaatttcaa gattcgccac aatgttgagg acggtagcgt ccaactggcc
     1921 gaccattacc agcagaacac cccaattggt gacggtccgg ttttgctgcc ggataatcac
     1981 tatctgagca cccaaagcgt gctgagcaaa gatccgaacg aaaaacgtga tcacatggtc
     2041 ctgctggaat ttgtgaccgc tgcgggcatc acccacggta tggacgagct gtataagtaa
     2101 tga
//