LOCUS       BBa_K1149011            2295 bp    DNA     linear       23-May-2024
DEFINITION  phaz2 expression
ACCESSION   BBa_K1149011
VERSION     BBa_K1149011.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..222
                     /label=BBa_K1149023
     misc_feature    1525..1551
                     /label=flag+2stop
     CDS             223..1551
                     /label=BBa_K1149038
     RBS             1552..1575
                     /label=rbs
     CDS             1576..2295
                     /label=sfGFP
     misc_feature    1552..2295
                     /label=BBa_K515005
     misc_feature    157..2295
                     /label=BBa_S05223
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aatacctgct gccgaccgct
      181 gctgctggtc tgctgctcct cgctgcccag ccggcgatgg ccatgatgtc aagtcagacc
      241 acacaatctt cgaaattctc gctgttcctc aagcgcgggc ttctccttgc ggcagcaccc
      301 ttgctggcaa tgagcgcatc ctcggctctc gctgcgaccc aggtaaccgg cttcggctcg
      361 aaccccggca atcttctgat gtacaagcat gtgccgtcca gcatgccggc caatgcgccg
      421 ctggtcatcg ccatgcatgg ttgcacccag agcgcctctg cctatgaagc caccggctgg
      481 acccagctcg ccaacaccta caagttctat gtggtgtatc cggaacagca aagcagcaat
      541 aatcagaaca agtgcttcaa ctggttcgag cccggcgaca tcgcgcgcgg ccagggcgaa
      601 gccctgtcga tcaagcaaat ggtcgacaag atgaaggcag accactcgat cgatacgaat
      661 cgtgtgtatg tcacaggcct gtcggcgggc ggttacatgg tcaacgtcat gttggctact
      721 tatcccgacg tctttgccgg cggcgcgcct ttttcgggcg gtccgtacaa ctgcgcgact
      781 tcgatgacca atgcgttcac ctgcatgagc ccgggcgtgg ataaaactcc ggctgcctgg
      841 ggcgacctgg cgcgcggcgg ctacagcggc tataccggcc gtaagccgat cgtatcgatc
      901 tggcacggcg atgctgacta taccgtcaag caatccaatc aggtcgaaga agtggagcag
      961 tggaccaact accatggcat cgaccaaacc gcagacgtaa gcgacacggt ggccggcttc
     1021 ccgcacaagg tgtacaagga tgccagcggt aatgcgctcg tggaaaccta taccatcacc
     1081 ggcatgggtc atggcactcc ggttgatccg ggcaccggct ccctgcaatg cggcaccgct
     1141 ggcgcctata tcctcgatgt gaatatctgc tcgagctatt acgttgccaa gttctgggga
     1201 ttgatcggcg gtagcggcac caccacgacc acgtcggctg gcaccactac gactacgtcg
     1261 gcaggaacca ccacgactaa ggcttcaacc acgaccacca aggtttccac tacaactact
     1321 gcatccacca cgacgacaac tgcgggcgct tgctacaact cgagcaacta cgcgcacgtg
     1381 acggctggtc gtgctcatga caccggtggc tatgcatata ccaacggttc gaaccagaag
     1441 atggggctga acaacacctt ctacaccagc aagctgcgta agaccggtac caattactac
     1501 gtcatcgaca ctacttgccc ggactacaag gatgacgacg acaagtaata aaaatcaaat
     1561 taaggaggta agataatgcg taaaggcgaa gaactgttca cgggcgtagt tccgattctg
     1621 gtcgagctgg acggcgatgt gaacggtcat aagtttagcg ttcgcggtga aggtgagggc
     1681 gacgcgacca acggcaaact gaccctgaag ttcatctgca ccaccggtaa actgccggtg
     1741 ccttggccga ccttggtgac gacgttgacg tatggcgtgc agtgttttgc gcgttatccg
     1801 gaccacatga aacaacacga tttcttcaaa tctgcgatgc cggagggtta cgtccaggag
     1861 cgtaccattt ccttcaagga tgatggctac tacaaaactc gcgcagaggt taagtttgaa
     1921 ggtgacacgc tggtcaatcg tatcgaattg aagggtatcg actttaaaga ggatggtaac
     1981 attctgggcc ataaactgga gtataacttc aacagccata atgtttacat tacggcagac
     2041 aagcaaaaga acggcatcaa ggccaatttc aagattcgcc acaatgttga ggacggtagc
     2101 gtccaactgg ccgaccatta ccagcagaac accccaattg gtgacggtcc ggttttgctg
     2161 ccggataatc actatctgag cacccaaagc gtgctgagca aagatccgaa cgaaaaacgt
     2221 gatcacatgg tcctgctgga atttgtgacc gctgcgggca tcacccacgg tatggacgag
     2281 ctgtataagt aatga
//