LOCUS       BBa_K1149013            1767 bp    DNA     linear       23-May-2024
DEFINITION  bdh2: converts 3HB to acetoacetate
ACCESSION   BBa_K1149013
VERSION     BBa_K1149013.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..222
                     /label=BBa_K1149022
     misc_feature    991..1023
                     /label=flag tag
     CDS             223..1023
                     /label=BBa_K1149039
     RBS             1024..1047
                     /label=rbs
     CDS             1048..1767
                     /label=sfGFP
     misc_feature    1024..1767
                     /label=BBa_K515005
     misc_feature    157..1767
                     /label=BBa_S05202
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aatatctgct gccgaccgca
      181 gcagcgggtc tgctgctgct ggcagcacag cctgcaatgg caatgctgca aggtaaaacc
      241 gcactggtta ccggtagcac ctgtggtatt ggtctgggta ttgcacaggc actggcagcc
      301 cagggtgcaa acattattgt taatggtttt cgtcgtgcag atggtgcacg tcagcagatt
      361 gcagcagccg gtcaggttat tcgtctgggt tatcatggtg cagatatgag caaagcaagc
      421 gaaattgaag atatgatgcg ttatgcagaa gcagaatttg cagccgatat tctggttaat
      481 aatgccggta ttcagcatgt tgcaagcatc gaagattttc cgcctgaacg ttgggatgca
      541 attattgcaa ttaatctgac cagcgcattt cataccaccc gtctggcact gcctggtatg
      601 cgtcagaaaa attggggtcg tgttattaac attgcaagca cccatggtct ggttgcaagc
      661 gcacagaaaa gcgcatatgt tgcagcaaaa catggtattg ttggtctgac caaagttaca
      721 gcactggaaa ccgcacagaa tcgtgttacc gcaaatgcaa tttgtccggg ttgggttctg
      781 acaccgctgg ttcagaaaca ggttcaggca cgtccggcac atggcattag cgttgaacag
      841 gcaaaacgtg aactggtgat tgaaaaacag ccgagcggtc agtttgttac accggatgaa
      901 ctgggtgcac tggccgtttt tctggcaagc gaagcaggtc gtcaggttcg tggtgcaatt
      961 tggaatatgg caggcggttg gtttgcacag gattataaag atgatgacga caaaatctaa
     1021 tgaaaatcaa attaaggagg taagataatg cgtaaaggcg aagaactgtt cacgggcgta
     1081 gttccgattc tggtcgagct ggacggcgat gtgaacggtc ataagtttag cgttcgcggt
     1141 gaaggtgagg gcgacgcgac caacggcaaa ctgaccctga agttcatctg caccaccggt
     1201 aaactgccgg tgccttggcc gaccttggtg acgacgttga cgtatggcgt gcagtgtttt
     1261 gcgcgttatc cggaccacat gaaacaacac gatttcttca aatctgcgat gccggagggt
     1321 tacgtccagg agcgtaccat ttccttcaag gatgatggct actacaaaac tcgcgcagag
     1381 gttaagtttg aaggtgacac gctggtcaat cgtatcgaat tgaagggtat cgactttaaa
     1441 gaggatggta acattctggg ccataaactg gagtataact tcaacagcca taatgtttac
     1501 attacggcag acaagcaaaa gaacggcatc aaggccaatt tcaagattcg ccacaatgtt
     1561 gaggacggta gcgtccaact ggccgaccat taccagcaga acaccccaat tggtgacggt
     1621 ccggttttgc tgccggataa tcactatctg agcacccaaa gcgtgctgag caaagatccg
     1681 aacgaaaaac gtgatcacat ggtcctgctg gaatttgtga ccgctgcggg catcacccac
     1741 ggtatggacg agctgtataa gtaatga
//