LOCUS       BBa_K115014             1339 bp    DNA     linear       17-May-2024
DEFINITION  Thermosensitive experimental expression
ACCESSION   BBa_K115014
VERSION     BBa_K115014.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     stem_loop       209..226
                     /label=Predicted stem loop
     stem_loop       232..260
                     /label=Predicted stem loop
     RBS             255..260
                     /label=SD
     misc_feature    231..236
                     /label=Scar adaptation
     misc_feature    209..260
                     /label=BBa_K115009
     misc_feature    267..1202
                     /label=Rluc
     CDS             267..1202
                     /label=Rluc
     misc_feature    267..1202
                     /label=BBa_J52008
     stem_loop       1222..1265
                     /label=stem_loop
     misc_feature    1211..1290
                     /label=BBa_B0010
     terminator      1211..1290
                     /label=BBa_B0010
     stem_loop       1306..1325
                     /label=T7 TE
     misc_feature    1332..1332
                     /label=stop
     polyA_site      1326..1339
                     /label=polya
     misc_feature    1299..1339
                     /label=BBa_B0012
     terminator      1299..1339
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg acaagcaatg cttgccttga atggtaactt
      241 ttgaatagtg attcaggagg tactagatgg cttccaaggt gtacgacccc gagcaacgca
      301 aacgcatgat cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac gtgctggact
      361 ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt tttctgcatg
      421 gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag cccgtggcta
      481 gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg aatggctcat
      541 atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg aaccttccaa
      601 agaaaatcat ctttgtgggc cacgactggg gggcttgtct ggcctttcac tactcctacg
      661 agcaccaaga caagatcaag gccatcgtcc atgctgagag tgtcgtggac gtgatcgagt
      721 cctgggacga gtggcctgac atcgaggagg atatcgccct gatcaagagc gaagagggcg
      781 agaaaatggt gcttgagaat aacttcttcg tcgagaccat gctcccaagc aagatcatgc
      841 ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag aagggcgagg
      901 ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaaggga ggcaagcccg
      961 acgtcgtcca gattgtccgc aactacaacg cctaccttcg ggccagcgac gatctgccta
     1021 agatgttcat cgagtccgac cctgggttct tttccaacgc tattgtcgag ggagctaaga
     1081 agttccctaa caccgagttc gtgaaggtga agggcctcca cttcagccag gaggacgctc
     1141 cagatgaaat gggtaagtac atcaagagct tcgtggagcg cgtgctgaag aacgagcagt
     1201 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1261 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1321 gggcctttct gcgtttata
//