LOCUS       BBa_K115015             1302 bp    DNA     linear       17-May-2024
DEFINITION  Reference expression of Renilla Luciferase
ACCESSION   BBa_K115015
VERSION     BBa_K115015.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    230..1165
                     /label=Rluc
     CDS             230..1165
                     /label=Rluc
     misc_feature    230..1165
                     /label=BBa_J52008
     stem_loop       1185..1228
                     /label=stem_loop
     misc_feature    1174..1253
                     /label=BBa_B0010
     terminator      1174..1253
                     /label=BBa_B0010
     stem_loop       1269..1288
                     /label=T7 TE
     misc_feature    1295..1295
                     /label=stop
     polyA_site      1289..1302
                     /label=polya
     misc_feature    1262..1302
                     /label=BBa_B0012
     terminator      1262..1302
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggcttccaa
      241 ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg
      301 caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga agcacgccga
      361 gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt
      421 gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc
      481 cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg
      541 gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg
      601 tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga
      661 gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc
      721 cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac
      781 catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga
      841 gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc
      901 tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct
      961 tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa
     1021 cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct
     1081 ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga
     1141 gcgcgtgctg aagaacgagc agtaatacta gagccaggca tcaaataaaa cgaaaggctc
     1201 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1261 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//