LOCUS       BBa_K115023             1188 bp    DNA     linear       17-May-2024
DEFINITION  construction of K115003 expression
ACCESSION   BBa_K115023
VERSION     BBa_K115023.1  
FEATURES             Location/Qualifiers
     RBS             106..109
                     /label=SD
     stem_loop       28..109
                     /label=Predicted stem loop up to A of AUG
     misc_feature    1..109
                     /label=BBa_K115003
     misc_feature    116..1051
                     /label=Rluc
     CDS             116..1051
                     /label=Rluc
     misc_feature    116..1051
                     /label=BBa_J52008
     stem_loop       1071..1114
                     /label=stem_loop
     misc_feature    1060..1139
                     /label=BBa_B0010
     terminator      1060..1139
                     /label=BBa_B0010
     stem_loop       1155..1174
                     /label=T7 TE
     misc_feature    1181..1181
                     /label=stop
     polyA_site      1175..1188
                     /label=polya
     misc_feature    1148..1188
                     /label=BBa_B0012
     terminator      1148..1188
                     /label=BBa_B0012
ORIGIN
        1 tgtaaaaaat attatttagc gtgactttct agtaacagct aacaattgtt gttactgcct
       61 aatgttttta gggtatttta aaaaagggcg ataaaaaacg attgggggat actagatggc
      121 ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc
      181 tcgctgcaag caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca
      241 cgccgagaac gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca
      301 cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg
      361 taagtccggc aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac
      421 cgcttggttc gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg
      481 ggcttgtctg gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca
      541 tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga
      601 tatcgccctg atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt
      661 cgagaccatg ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta
      721 cctggagcca ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga
      781 gatccctctc gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc
      841 ctaccttcgg gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt
      901 ttccaacgct attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa
      961 gggcctccac ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt
     1021 cgtggagcgc gtgctgaaga acgagcagta atactagagc caggcatcaa ataaaacgaa
     1081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1141 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//