LOCUS       BBa_K115027             1162 bp    DNA     linear       17-May-2024
DEFINITION  construction of K115017 expression
ACCESSION   BBa_K115027
VERSION     BBa_K115027.1  
FEATURES             Location/Qualifiers
     RBS             77..82
                     /label=SD
     misc_feature    1..83
                     /label=BBa_K115017
     misc_feature    90..1025
                     /label=Rluc
     CDS             90..1025
                     /label=Rluc
     misc_feature    90..1025
                     /label=BBa_J52008
     stem_loop       1045..1088
                     /label=stem_loop
     misc_feature    1034..1113
                     /label=BBa_B0010
     terminator      1034..1113
                     /label=BBa_B0010
     stem_loop       1129..1148
                     /label=T7 TE
     misc_feature    1155..1155
                     /label=stop
     polyA_site      1149..1162
                     /label=polya
     misc_feature    1122..1162
                     /label=BBa_B0012
     terminator      1122..1162
                     /label=BBa_B0012
ORIGIN
        1 ccgggcgccc ttcgggggcc cggcggagac gggcgccgga ggtgtccgac gcctgctcgt
       61 ccagtctttg ctcagtggag gattactaga tggcttccaa ggtgtacgac cccgagcaac
      121 gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg
      181 actccttcat caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc
      241 atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg
      301 ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct
      361 catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc
      421 caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct
      481 acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg
      541 agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg
      601 gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca
      661 tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg
      721 aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc
      781 ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc
      841 ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta
      901 agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg
      961 ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc
     1021 agtaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1081 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1141 ggtgggcctt tctgcgttta ta
//