LOCUS       BBa_K1151009            1011 bp    DNA     linear       09-May-2024
DEFINITION  GFP nickel regulated 1
ACCESSION   BBa_K1151009
VERSION     BBa_K1151009.1  
FEATURES             Location/Qualifiers
     promoter        1..127
                     /label=BBa_K1151003
     RBS             136..148
                     /label=RBS-3\Weak
     RBS             142..145
                     /label=RBS
     misc_feature    136..148
                     /label=BBa_B0032
     RBS             136..148
                     /label=BBa_B0032
     CDS             155..874
                     /label=GFP protein
     CDS             155..874
                     /label=BBa_E0040
     stem_loop       894..937
                     /label=stem_loop
     misc_feature    883..962
                     /label=BBa_B0010
     terminator      883..962
                     /label=BBa_B0010
     stem_loop       978..997
                     /label=T7 TE
     misc_feature    1004..1004
                     /label=stop
     polyA_site      998..1011
                     /label=polya
     misc_feature    971..1011
                     /label=BBa_B0012
     terminator      971..1011
                     /label=BBa_B0012
     misc_feature    136..1011
                     /label=BBa_E0240
ORIGIN
        1 aattcaaacg ctcttatggc caaccattta aatccagttt gtattataat tgttaatttt
       61 aaattaattc aatcatacta gcatgagcgg cttttaacga actcatgcca aaaaaggatt
      121 tttctcatac tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac
      181 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      241 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      301 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
      361 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      421 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      481 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      541 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      601 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      661 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      721 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
      781 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
      841 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
      901 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      961 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//