LOCUS       BBa_K1151011            1132 bp    DNA     linear       01-Jun-2024
DEFINITION  GFP nickel regulated 3
ACCESSION   BBa_K1151011
VERSION     BBa_K1151011.1  
FEATURES             Location/Qualifiers
     promoter        1..248
                     /label=BBa_K1151002
     RBS             257..269
                     /label=RBS-3\Weak
     RBS             263..266
                     /label=RBS
     misc_feature    257..269
                     /label=BBa_B0032
     RBS             257..269
                     /label=BBa_B0032
     CDS             276..995
                     /label=GFP protein
     CDS             276..995
                     /label=BBa_E0040
     stem_loop       1015..1058
                     /label=stem_loop
     misc_feature    1004..1083
                     /label=BBa_B0010
     terminator      1004..1083
                     /label=BBa_B0010
     stem_loop       1099..1118
                     /label=T7 TE
     misc_feature    1125..1125
                     /label=stop
     polyA_site      1119..1132
                     /label=polya
     misc_feature    1092..1132
                     /label=BBa_B0012
     terminator      1092..1132
                     /label=BBa_B0012
     misc_feature    257..1132
                     /label=BBa_E0240
ORIGIN
        1 tgagaaaaat ccttttttgg catgagttcg ttaaaagccg ctcatgctag tatgattgaa
       61 ttaatttaaa attaacaatt ataatacaaa ctggatttaa atggttggcc ataagagcgt
      121 ttgaatttga ttgtaattat tagcttaatc atcattgact tgttattatt aaaacaatac
      181 aatcaacaaa ccaacattct tttatcattt tggagcattt atgcgaacgg atgcttctta
      241 tagggtgcta ctagagtcac acaggaaagt actagatgcg taaaggagaa gaacttttca
      301 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      361 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      421 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
      481 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
      541 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
      601 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
      661 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
      721 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
      781 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
      841 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
      901 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
      961 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
     1021 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1081 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//