LOCUS BBa_K1151020 3962 bp DNA linear 28-May-2024 DEFINITION LuxI-HpNikR-GFP-RFP 1 ACCESSION BBa_K1151020 VERSION BBa_K1151020.1 FEATURES Location/Qualifiers promoter 1..253 /label=BBa_K1151005 misc_feature 266..269 /label=conserved RBS 262..273 /label=BBa_B0034 misc_feature 859..890 /label=LVA CDS 280..858 /label=luxI misc_feature 280..897 /label=BBa_C0061 misc_feature 898..922 /label=Help:Barcodes CDS 280..897 /label=BBa_C0061 stem_loop 942..985 /label=stem_loop misc_feature 931..1010 /label=BBa_B0010 terminator 931..1010 /label=BBa_B0010 stem_loop 1026..1045 /label=T7 TE misc_feature 1052..1052 /label=stop polyA_site 1046..1059 /label=polya misc_feature 1019..1059 /label=BBa_B0012 terminator 1019..1059 /label=BBa_B0012 terminator 931..1059 /label=BBa_B0015 misc_feature 262..1059 /label=BBa_K805016 misc_feature 1..1059 /label=BBa_K1151012 CDS 1068..1155 /label=end of LacI coding region (inactive) misc_feature 1068..1267 /label=BBa_R0010 misc_feature 1156..1193 /label=CAP binding site promoter 1204..1209 /label=-35 promoter 1228..1233 /label=-10 misc_feature 1233..1267 /label=LacI binding site misc_feature 1240..1240 /label=start promoter 1068..1267 /label=BBa_R0010 misc_feature 1280..1283 /label=conserved RBS 1276..1287 /label=BBa_B0034 misc_feature 1068..1287 /label=BBa_J04500 CDS 1294..1740 /label=BBa_K1151000 stem_loop 1760..1803 /label=stem_loop misc_feature 1749..1828 /label=BBa_B0010 terminator 1749..1828 /label=BBa_B0010 stem_loop 1844..1863 /label=T7 TE misc_feature 1870..1870 /label=stop polyA_site 1864..1877 /label=polya misc_feature 1837..1877 /label=BBa_B0012 terminator 1837..1877 /label=BBa_B0012 terminator 1749..1877 /label=BBa_B0015 misc_feature 1068..1877 /label=BBa_K1151006 promoter 1886..2044 /label=BBa_K1151004 RBS 2053..2065 /label=RBS-3\Weak RBS 2059..2062 /label=RBS misc_feature 2053..2065 /label=BBa_B0032 RBS 2053..2065 /label=BBa_B0032 CDS 2072..2791 /label=GFP protein CDS 2072..2791 /label=BBa_E0040 stem_loop 2811..2854 /label=stem_loop misc_feature 2800..2879 /label=BBa_B0010 terminator 2800..2879 /label=BBa_B0010 stem_loop 2895..2914 /label=T7 TE misc_feature 2921..2921 /label=stop polyA_site 2915..2928 /label=polya misc_feature 2888..2928 /label=BBa_B0012 terminator 2888..2928 /label=BBa_B0012 misc_feature 2053..2928 /label=BBa_E0240 misc_feature 1886..2928 /label=BBa_K1151010 promoter 2937..3189 /label=BBa_K1151005 RBS 3198..3209 /label=B0034 CDS 3216..3962 /label=mCherry-LVA misc_feature 3216..3218 /label=Start misc_feature 3927..3956 /label=ssrA degradation tag: LVA misc_feature 3957..3959 /label=Stop misc_feature 3960..3962 /label=Stop misc_feature 3198..3962 /label=BBa_K773003 misc_feature 2937..3962 /label=BBa_K1151008 ORIGIN 1 ctcattctcc tattcttaaa gtgtttttcc ttgaagacat aacgaaatca aggttggatg 61 taattgtagc aatgttttga tttactaaga ttaacagaag cgttcattaa ctaattatta 121 tttaaaatga attagtgtta tatttttgaa gcgctataaa agcgttatta gaagcgcgtt 181 taatacccct ttagaattta taaagatcaa aaactcgcaa aaaaaatgag aattaaaatt 241 ggagtgataa tggtactaga gaaagaggag aaatactaga tgactataat gataaaaaaa 301 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa 361 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag 421 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga 481 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg 541 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta 601 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa 661 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca 721 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa 781 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt 841 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 901 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1021 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1141 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 1201 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 1261 tcacacatac tagagaaaga ggagaaatac tagatggata cacccaataa agacgattca 1321 atcatccgct tttcggtttc tttacaacaa aatttattag acgaattaga caaccgcatc 1381 attaaaaacg gctattcttc tcgctcagaa ttagtgcgcg acatgatcag agaaaaatta 1441 gtagaagaca attgggccga agataaccct aatgatgaga gcaaaatcgc cgtgcttgtg 1501 gtgatttatg atcaccacca aagggaattg aaccagcgca tgatagacat tcagcatgcc 1561 agcgggacgc atgttttatg caccactcac attcacatgg atgagcataa ttgtttagag 1621 acgattattt tacaaggcaa ttcgtttgaa atccaacgct tgcaactaga aattgggggg 1681 cttagggggg ttaaattcgc taaattgact aaggcgtcta gctttgaaca caatgaatag 1741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1861 gcctttctgc gtttatatac tagagactgg atttaaatgg ttggccataa gagcgtttga 1921 atttgattgt aattattagc ttaatcatca ttgacttgtt attattaaaa caatacaatc 1981 aacaaaccaa cattctttta tcattttgga gcatttatgc gaacggatgc ttcttatagg 2041 gtgctactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg 2101 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 2161 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 2221 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 2281 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 2341 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 2401 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 2461 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 2521 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 2581 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 2641 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 2701 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 2761 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa 2821 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2881 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagctca 2941 ttctcctatt cttaaagtgt ttttccttga agacataacg aaatcaaggt tggatgtaat 3001 tgtagcaatg ttttgattta ctaagattaa cagaagcgtt cattaactaa ttattattta 3061 aaatgaatta gtgttatatt tttgaagcgc tataaaagcg ttattagaag cgcgtttaat 3121 acccctttag aatttataaa gatcaaaaac tcgcaaaaaa aatgagaatt aaaattggag 3181 tgataatggt actagagaaa gaggagaaat actagatggt gagcaagggc gaggaggata 3241 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg 3301 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 3361 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc 3421 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga 3481 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 3541 tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac aaggtgaagc 3601 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg 3661 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga 3721 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca 3781 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc 3841 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg 3901 gcggcatgga cgagctgtac aaggcagcaa acgacgaaaa ctacgctcta gtagcttaat 3961 aa //