LOCUS BBa_K1151021 3930 bp DNA linear 29-May-2024 DEFINITION LuxI-HpNikR-GFP-RFP 2 ACCESSION BBa_K1151021 VERSION BBa_K1151021.1 FEATURES Location/Qualifiers promoter 1..253 /label=BBa_K1151005 misc_feature 266..269 /label=conserved RBS 262..273 /label=BBa_B0034 misc_feature 859..890 /label=LVA CDS 280..858 /label=luxI misc_feature 280..897 /label=BBa_C0061 misc_feature 898..922 /label=Help:Barcodes CDS 280..897 /label=BBa_C0061 stem_loop 942..985 /label=stem_loop misc_feature 931..1010 /label=BBa_B0010 terminator 931..1010 /label=BBa_B0010 stem_loop 1026..1045 /label=T7 TE misc_feature 1052..1052 /label=stop polyA_site 1046..1059 /label=polya misc_feature 1019..1059 /label=BBa_B0012 terminator 1019..1059 /label=BBa_B0012 terminator 931..1059 /label=BBa_B0015 misc_feature 262..1059 /label=BBa_K805016 misc_feature 1..1059 /label=BBa_K1151012 CDS 1068..1155 /label=end of LacI coding region (inactive) misc_feature 1068..1267 /label=BBa_R0010 misc_feature 1156..1193 /label=CAP binding site promoter 1204..1209 /label=-35 promoter 1228..1233 /label=-10 misc_feature 1233..1267 /label=LacI binding site misc_feature 1240..1240 /label=start promoter 1068..1267 /label=BBa_R0010 misc_feature 1280..1283 /label=conserved RBS 1276..1287 /label=BBa_B0034 misc_feature 1068..1287 /label=BBa_J04500 CDS 1294..1740 /label=BBa_K1151000 stem_loop 1760..1803 /label=stem_loop misc_feature 1749..1828 /label=BBa_B0010 terminator 1749..1828 /label=BBa_B0010 stem_loop 1844..1863 /label=T7 TE misc_feature 1870..1870 /label=stop polyA_site 1864..1877 /label=polya misc_feature 1837..1877 /label=BBa_B0012 terminator 1837..1877 /label=BBa_B0012 terminator 1749..1877 /label=BBa_B0015 misc_feature 1068..1877 /label=BBa_K1151006 misc_feature 1..1877 /label=BBa_K1151016 promoter 1886..2012 /label=BBa_K1151003 RBS 2021..2033 /label=RBS-3\Weak RBS 2027..2030 /label=RBS misc_feature 2021..2033 /label=BBa_B0032 RBS 2021..2033 /label=BBa_B0032 CDS 2040..2759 /label=GFP protein CDS 2040..2759 /label=BBa_E0040 stem_loop 2779..2822 /label=stem_loop misc_feature 2768..2847 /label=BBa_B0010 terminator 2768..2847 /label=BBa_B0010 stem_loop 2863..2882 /label=T7 TE misc_feature 2889..2889 /label=stop polyA_site 2883..2896 /label=polya misc_feature 2856..2896 /label=BBa_B0012 terminator 2856..2896 /label=BBa_B0012 misc_feature 2021..2896 /label=BBa_E0240 misc_feature 1886..2896 /label=BBa_K1151009 promoter 2905..3157 /label=BBa_K1151005 RBS 3166..3177 /label=B0034 CDS 3184..3930 /label=mCherry-LVA misc_feature 3184..3186 /label=Start misc_feature 3895..3924 /label=ssrA degradation tag: LVA misc_feature 3925..3927 /label=Stop misc_feature 3928..3930 /label=Stop misc_feature 3166..3930 /label=BBa_K773003 misc_feature 2905..3930 /label=BBa_K1151008 misc_feature 1886..3930 /label=BBa_K1151017 ORIGIN 1 ctcattctcc tattcttaaa gtgtttttcc ttgaagacat aacgaaatca aggttggatg 61 taattgtagc aatgttttga tttactaaga ttaacagaag cgttcattaa ctaattatta 121 tttaaaatga attagtgtta tatttttgaa gcgctataaa agcgttatta gaagcgcgtt 181 taatacccct ttagaattta taaagatcaa aaactcgcaa aaaaaatgag aattaaaatt 241 ggagtgataa tggtactaga gaaagaggag aaatactaga tgactataat gataaaaaaa 301 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa 361 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag 421 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga 481 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg 541 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta 601 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa 661 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca 721 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa 781 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt 841 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 901 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1021 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1141 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 1201 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 1261 tcacacatac tagagaaaga ggagaaatac tagatggata cacccaataa agacgattca 1321 atcatccgct tttcggtttc tttacaacaa aatttattag acgaattaga caaccgcatc 1381 attaaaaacg gctattcttc tcgctcagaa ttagtgcgcg acatgatcag agaaaaatta 1441 gtagaagaca attgggccga agataaccct aatgatgaga gcaaaatcgc cgtgcttgtg 1501 gtgatttatg atcaccacca aagggaattg aaccagcgca tgatagacat tcagcatgcc 1561 agcgggacgc atgttttatg caccactcac attcacatgg atgagcataa ttgtttagag 1621 acgattattt tacaaggcaa ttcgtttgaa atccaacgct tgcaactaga aattgggggg 1681 cttagggggg ttaaattcgc taaattgact aaggcgtcta gctttgaaca caatgaatag 1741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1861 gcctttctgc gtttatatac tagagaattc aaacgctctt atggccaacc atttaaatcc 1921 agtttgtatt ataattgtta attttaaatt aattcaatca tactagcatg agcggctttt 1981 aacgaactca tgccaaaaaa ggatttttct catactagag tcacacagga aagtactaga 2041 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg 2101 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa 2161 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg 2221 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc 2281 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca 2341 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta 2401 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat 2461 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa 2521 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc 2581 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc 2641 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc 2701 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat 2761 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 2821 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 2881 cctttctgcg tttatatact agagctcatt ctcctattct taaagtgttt ttccttgaag 2941 acataacgaa atcaaggttg gatgtaattg tagcaatgtt ttgatttact aagattaaca 3001 gaagcgttca ttaactaatt attatttaaa atgaattagt gttatatttt tgaagcgcta 3061 taaaagcgtt attagaagcg cgtttaatac ccctttagaa tttataaaga tcaaaaactc 3121 gcaaaaaaaa tgagaattaa aattggagtg ataatggtac tagagaaaga ggagaaatac 3181 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc 3241 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag 3301 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg 3361 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag 3421 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag 3481 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag 3541 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 3601 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac 3661 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac 3721 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac 3781 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 3841 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa ggcagcaaac 3901 gacgaaaact acgctctagt agcttaataa //