LOCUS BBa_K1151022 4051 bp DNA linear 30-May-2024 DEFINITION LuxI-HpNikR-GFP-RFP 3 ACCESSION BBa_K1151022 VERSION BBa_K1151022.1 FEATURES Location/Qualifiers promoter 1..253 /label=BBa_K1151005 misc_feature 266..269 /label=conserved RBS 262..273 /label=BBa_B0034 misc_feature 859..890 /label=LVA CDS 280..858 /label=luxI misc_feature 280..897 /label=BBa_C0061 misc_feature 898..922 /label=Help:Barcodes CDS 280..897 /label=BBa_C0061 stem_loop 942..985 /label=stem_loop misc_feature 931..1010 /label=BBa_B0010 terminator 931..1010 /label=BBa_B0010 stem_loop 1026..1045 /label=T7 TE misc_feature 1052..1052 /label=stop polyA_site 1046..1059 /label=polya misc_feature 1019..1059 /label=BBa_B0012 terminator 1019..1059 /label=BBa_B0012 terminator 931..1059 /label=BBa_B0015 misc_feature 262..1059 /label=BBa_K805016 misc_feature 1..1059 /label=BBa_K1151012 CDS 1068..1155 /label=end of LacI coding region (inactive) misc_feature 1068..1267 /label=BBa_R0010 misc_feature 1156..1193 /label=CAP binding site promoter 1204..1209 /label=-35 promoter 1228..1233 /label=-10 misc_feature 1233..1267 /label=LacI binding site misc_feature 1240..1240 /label=start promoter 1068..1267 /label=BBa_R0010 misc_feature 1280..1283 /label=conserved RBS 1276..1287 /label=BBa_B0034 misc_feature 1068..1287 /label=BBa_J04500 CDS 1294..1740 /label=BBa_K1151000 stem_loop 1760..1803 /label=stem_loop misc_feature 1749..1828 /label=BBa_B0010 terminator 1749..1828 /label=BBa_B0010 stem_loop 1844..1863 /label=T7 TE misc_feature 1870..1870 /label=stop polyA_site 1864..1877 /label=polya misc_feature 1837..1877 /label=BBa_B0012 terminator 1837..1877 /label=BBa_B0012 terminator 1749..1877 /label=BBa_B0015 misc_feature 1068..1877 /label=BBa_K1151006 misc_feature 1..1877 /label=BBa_K1151016 promoter 1886..2133 /label=BBa_K1151002 RBS 2142..2154 /label=RBS-3\Weak RBS 2148..2151 /label=RBS misc_feature 2142..2154 /label=BBa_B0032 RBS 2142..2154 /label=BBa_B0032 CDS 2161..2880 /label=GFP protein CDS 2161..2880 /label=BBa_E0040 stem_loop 2900..2943 /label=stem_loop misc_feature 2889..2968 /label=BBa_B0010 terminator 2889..2968 /label=BBa_B0010 stem_loop 2984..3003 /label=T7 TE misc_feature 3010..3010 /label=stop polyA_site 3004..3017 /label=polya misc_feature 2977..3017 /label=BBa_B0012 terminator 2977..3017 /label=BBa_B0012 misc_feature 2142..3017 /label=BBa_E0240 misc_feature 1886..3017 /label=BBa_K1151011 promoter 3026..3278 /label=BBa_K1151005 RBS 3287..3298 /label=B0034 CDS 3305..4051 /label=mCherry-LVA misc_feature 3305..3307 /label=Start misc_feature 4016..4045 /label=ssrA degradation tag: LVA misc_feature 4046..4048 /label=Stop misc_feature 4049..4051 /label=Stop misc_feature 3287..4051 /label=BBa_K773003 misc_feature 3026..4051 /label=BBa_K1151008 misc_feature 1886..4051 /label=BBa_K1151019 ORIGIN 1 ctcattctcc tattcttaaa gtgtttttcc ttgaagacat aacgaaatca aggttggatg 61 taattgtagc aatgttttga tttactaaga ttaacagaag cgttcattaa ctaattatta 121 tttaaaatga attagtgtta tatttttgaa gcgctataaa agcgttatta gaagcgcgtt 181 taatacccct ttagaattta taaagatcaa aaactcgcaa aaaaaatgag aattaaaatt 241 ggagtgataa tggtactaga gaaagaggag aaatactaga tgactataat gataaaaaaa 301 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa 361 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag 421 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga 481 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg 541 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta 601 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa 661 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca 721 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa 781 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt 841 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 901 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1021 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1141 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 1201 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 1261 tcacacatac tagagaaaga ggagaaatac tagatggata cacccaataa agacgattca 1321 atcatccgct tttcggtttc tttacaacaa aatttattag acgaattaga caaccgcatc 1381 attaaaaacg gctattcttc tcgctcagaa ttagtgcgcg acatgatcag agaaaaatta 1441 gtagaagaca attgggccga agataaccct aatgatgaga gcaaaatcgc cgtgcttgtg 1501 gtgatttatg atcaccacca aagggaattg aaccagcgca tgatagacat tcagcatgcc 1561 agcgggacgc atgttttatg caccactcac attcacatgg atgagcataa ttgtttagag 1621 acgattattt tacaaggcaa ttcgtttgaa atccaacgct tgcaactaga aattgggggg 1681 cttagggggg ttaaattcgc taaattgact aaggcgtcta gctttgaaca caatgaatag 1741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1861 gcctttctgc gtttatatac tagagtgaga aaaatccttt tttggcatga gttcgttaaa 1921 agccgctcat gctagtatga ttgaattaat ttaaaattaa caattataat acaaactgga 1981 tttaaatggt tggccataag agcgtttgaa tttgattgta attattagct taatcatcat 2041 tgacttgtta ttattaaaac aatacaatca acaaaccaac attcttttat cattttggag 2101 catttatgcg aacggatgct tcttataggg tgctactaga gtcacacagg aaagtactag 2161 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 2221 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga 2281 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 2341 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag 2401 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc 2461 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt 2521 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa 2581 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga 2641 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac 2701 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 2761 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt 2821 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa 2881 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2941 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3001 gcctttctgc gtttatatac tagagctcat tctcctattc ttaaagtgtt tttccttgaa 3061 gacataacga aatcaaggtt ggatgtaatt gtagcaatgt tttgatttac taagattaac 3121 agaagcgttc attaactaat tattatttaa aatgaattag tgttatattt ttgaagcgct 3181 ataaaagcgt tattagaagc gcgtttaata cccctttaga atttataaag atcaaaaact 3241 cgcaaaaaaa atgagaatta aaattggagt gataatggta ctagagaaag aggagaaata 3301 ctagatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt 3361 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga 3421 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct 3481 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa 3541 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga 3601 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccttgca 3661 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc 3721 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga 3781 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga 3841 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa 3901 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta 3961 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcagcaaa 4021 cgacgaaaac tacgctctag tagcttaata a //