LOCUS       BBa_K1157014            2727 bp    DNA     linear       12-May-2024
DEFINITION  pLas-LasR-GFPmut3-Pc-LasR
ACCESSION   BBa_K1157014
VERSION     BBa_K1157014.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..180
                     /label=RBS-1\Strong
     RBS             173..177
                     /label=RBS
     misc_feature    166..180
                     /label=BBa_B0030
     RBS             166..180
                     /label=BBa_B0030
     CDS             187..909
                     /label=BBa_C0179
     RBS             918..932
                     /label=RBS-1\Strong
     RBS             925..929
                     /label=RBS
     misc_feature    918..932
                     /label=BBa_B0030
     RBS             918..932
                     /label=BBa_B0030
     CDS             939..1658
                     /label=GFP protein
     CDS             939..1658
                     /label=BBa_E0040
     stem_loop       1678..1721
                     /label=stem_loop
     misc_feature    1667..1746
                     /label=BBa_B0010
     terminator      1667..1746
                     /label=BBa_B0010
     stem_loop       1762..1781
                     /label=T7 TE
     misc_feature    1788..1788
                     /label=stop
     polyA_site      1782..1795
                     /label=polya
     misc_feature    1755..1795
                     /label=BBa_B0012
     terminator      1755..1795
                     /label=BBa_B0012
     terminator      1667..1795
                     /label=BBa_B0015
     promoter        1804..1838
                     /label=BBa_J23100
     RBS             1847..1861
                     /label=RBS-1\Strong
     RBS             1854..1858
                     /label=RBS
     misc_feature    1847..1861
                     /label=BBa_B0030
     RBS             1847..1861
                     /label=BBa_B0030
     CDS             1868..2590
                     /label=BBa_C0179
     stem_loop       2610..2653
                     /label=stem_loop
     misc_feature    2599..2678
                     /label=BBa_B0010
     terminator      2599..2678
                     /label=BBa_B0010
     stem_loop       2694..2713
                     /label=T7 TE
     misc_feature    2720..2720
                     /label=stop
     polyA_site      2714..2727
                     /label=polya
     misc_feature    2687..2727
                     /label=BBa_B0012
     terminator      2687..2727
                     /label=BBa_B0012
     terminator      2599..2727
                     /label=BBa_B0015
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa
      181 tactagatgg ccttggttga cggttttctt gagctggaac gctcaagtgg aaaattggag
      241 tggagcgcca tcctccagaa gatggcgagc gaccttggat tctcgaagat cctgttcggc
      301 ctgttgccta aggacagcca ggactacgag aacgccttca tcgtcggcaa ctacccggcc
      361 gcctggcgcg agcattacga ccgggctggc tacgcgcggg tcgacccgac ggtcagtcac
      421 tgtacccaga gcgtactgcc gattttctgg gaaccgtcca tctaccagac gcgaaagcag
      481 cacgagttct tcgaggaagc ctcggccgcc ggcctggtgt atgggctgac catgccgctg
      541 catggtgctc gcggcgaact cggcgcgctg agcctcagcg tggaagcgga aaaccgggcc
      601 gaggccaacc gtttcataga gtcggtcctg ccgaccctgt ggatgctcaa ggactacgca
      661 ctgcaaagcg gtgccggact ggccttcgaa catccggtca gcaaaccggt ggttctgacc
      721 agccgggaga aggaagtgtt gcagtggtgc gccatcggca agaccagttg ggagatatcg
      781 gttatctgca actgctcgga agccaatgtg aacttccata tgggaaatat tcggcggaag
      841 ttcggtgtga cctcccgccg cgtagcggcc attatggccg ttaatttggg tcttattact
      901 ctctaataat actagagatt aaagaggaga aatactagat gcgtaaagga gaagaacttt
      961 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     1021 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     1081 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
     1141 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
     1201 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     1261 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     1321 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     1381 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     1441 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     1501 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     1561 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     1621 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
     1681 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1741 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1801 gagttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa
     1861 atactagatg gccttggttg acggttttct tgagctggaa cgctcaagtg gaaaattgga
     1921 gtggagcgcc atcctccaga agatggcgag cgaccttgga ttctcgaaga tcctgttcgg
     1981 cctgttgcct aaggacagcc aggactacga gaacgccttc atcgtcggca actacccggc
     2041 cgcctggcgc gagcattacg accgggctgg ctacgcgcgg gtcgacccga cggtcagtca
     2101 ctgtacccag agcgtactgc cgattttctg ggaaccgtcc atctaccaga cgcgaaagca
     2161 gcacgagttc ttcgaggaag cctcggccgc cggcctggtg tatgggctga ccatgccgct
     2221 gcatggtgct cgcggcgaac tcggcgcgct gagcctcagc gtggaagcgg aaaaccgggc
     2281 cgaggccaac cgtttcatag agtcggtcct gccgaccctg tggatgctca aggactacgc
     2341 actgcaaagc ggtgccggac tggccttcga acatccggtc agcaaaccgg tggttctgac
     2401 cagccgggag aaggaagtgt tgcagtggtg cgccatcggc aagaccagtt gggagatatc
     2461 ggttatctgc aactgctcgg aagccaatgt gaacttccat atgggaaata ttcggcggaa
     2521 gttcggtgtg acctcccgcc gcgtagcggc cattatggcc gttaatttgg gtcttattac
     2581 tctctaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2641 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2701 cttcgggtgg gcctttctgc gtttata
//