LOCUS       BBa_K1157016            1960 bp    DNA     linear       11-May-2024
DEFINITION  Pc-LasR-pLas-BFP
ACCESSION   BBa_K1157016
VERSION     BBa_K1157016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..787
                     /label=BBa_C0179
     stem_loop       807..850
                     /label=stem_loop
     misc_feature    796..875
                     /label=BBa_B0010
     terminator      796..875
                     /label=BBa_B0010
     stem_loop       891..910
                     /label=T7 TE
     misc_feature    917..917
                     /label=stop
     polyA_site      911..924
                     /label=polya
     misc_feature    884..924
                     /label=BBa_B0012
     terminator      884..924
                     /label=BBa_B0012
     terminator      796..924
                     /label=BBa_B0015
     misc_feature    978..995
                     /label=OP2
     misc_feature    1038..1057
                     /label=OP1
     promoter        1049..1054
                     /label=-35
     promoter        1072..1077
                     /label=-10
     promoter        933..1089
                     /label=BBa_R0079
     RBS             1098..1112
                     /label=RBS-1\Strong
     RBS             1105..1109
                     /label=RBS
     misc_feature    1098..1112
                     /label=BBa_B0030
     RBS             1098..1112
                     /label=BBa_B0030
     CDS             1119..1817
                     /label=mTagBFP
     CDS             1119..1823
                     /label=BBa_K592100
     stem_loop       1843..1886
                     /label=stem_loop
     misc_feature    1832..1911
                     /label=BBa_B0010
     terminator      1832..1911
                     /label=BBa_B0010
     stem_loop       1927..1946
                     /label=T7 TE
     misc_feature    1953..1953
                     /label=stop
     polyA_site      1947..1960
                     /label=polya
     misc_feature    1920..1960
                     /label=BBa_B0012
     terminator      1920..1960
                     /label=BBa_B0012
     terminator      1832..1960
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatggcc ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg
      121 gagcgccatc ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct
      181 gttgcctaag gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc
      241 ctggcgcgag cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg
      301 tacccagagc gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca
      361 cgagttcttc gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca
      421 tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga
      481 ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact
      541 gcaaagcggt gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag
      601 ccgggagaag gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt
      661 tatctgcaac tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt
      721 cggtgtgacc tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct
      781 ctaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      841 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      901 cgggtgggcc tttctgcgtt tatatactag aggcccctcg ctgagcgcgt cccggagctg
      961 ggggcaacct agctgccacc tgcttttctg ctagctattc cagcgaaaac atacagattt
     1021 ccggcgaaat caaggctacc tgccagttct ggcaggtttg gccgcgggtt ctttttggta
     1081 cacgaaagct actagagatt aaagaggaga aatactagat gagcgaactg atcaaagaga
     1141 acatgcacat gaagctgtac atggaaggca ccgttgacaa ccaccacttt aagtgcacgt
     1201 ctgagggtga gggtaagccg tacgaaggca cccaaaccat gcgtatcaaa gttgtggagg
     1261 gcggtccact gccgttcgct tttgacattc tggcgaccag cttcctgtac ggttccaaaa
     1321 cgttcattaa ccatactcag ggcattccgg atttcttcaa acagagcttt ccggaaggtt
     1381 tcacctggga gcgtgtcacc acgtatgaag atggtggtgt gttgaccgcc acccaagata
     1441 cctccctgca agatggctgt ctgatctata acgtgaaaat tcgtggcgtc aactttacga
     1501 gcaatggtcc ggtgatgcag aagaaaaccc tgggttggga ggcgtttacg gaaaccctgt
     1561 atccggccga tggtggcctg gagggccgta acgacatggc actgaagctg gttggtggca
     1621 gccatttgat cgcaaatatc aagacgacgt accgcagcaa gaaaccggcg aaaaatctga
     1681 agatgccggg tgtttactat gtcgactacc gtctggaacg cattaaagaa gcgaataatg
     1741 agacttacgt ggagcagcac gaggttgcag tcgcgcgcta ttgcgacttg cctagcaagc
     1801 tgggtcataa actgaattaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1861 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1921 cacactggct caccttcggg tgggcctttc tgcgtttata
//