LOCUS       BBa_K1157018            2629 bp    DNA     linear       18-May-2024
DEFINITION  pRhl-RhlR-mCherry-Pc-RhlR
ACCESSION   BBa_K1157018
VERSION     BBa_K1157018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..76
                     /label=RBS-1\Strong
     RBS             69..73
                     /label=RBS
     misc_feature    62..76
                     /label=BBa_B0030
     RBS             62..76
                     /label=BBa_B0030
     CDS             83..811
                     /label=BBa_C0171
     RBS             820..834
                     /label=RBS-1\Strong
     RBS             827..831
                     /label=RBS
     misc_feature    820..834
                     /label=BBa_B0030
     RBS             820..834
                     /label=BBa_B0030
     CDS             841..1551
                     /label=mCherry
     misc_feature    1192..1192
                     /label=C->T (removing PstI site)
     misc_feature    841..1554
                     /label=BBa_J06504
     stem_loop       1574..1617
                     /label=stem_loop
     misc_feature    1563..1642
                     /label=BBa_B0010
     terminator      1563..1642
                     /label=BBa_B0010
     stem_loop       1658..1677
                     /label=T7 TE
     misc_feature    1684..1684
                     /label=stop
     polyA_site      1678..1691
                     /label=polya
     misc_feature    1651..1691
                     /label=BBa_B0012
     terminator      1651..1691
                     /label=BBa_B0012
     terminator      1563..1691
                     /label=BBa_B0015
     promoter        1700..1734
                     /label=BBa_J23100
     RBS             1743..1757
                     /label=RBS-1\Strong
     RBS             1750..1754
                     /label=RBS
     misc_feature    1743..1757
                     /label=BBa_B0030
     RBS             1743..1757
                     /label=BBa_B0030
     CDS             1764..2492
                     /label=BBa_C0171
     stem_loop       2512..2555
                     /label=stem_loop
     misc_feature    2501..2580
                     /label=BBa_B0010
     terminator      2501..2580
                     /label=BBa_B0010
     stem_loop       2596..2615
                     /label=T7 TE
     misc_feature    2622..2622
                     /label=stop
     polyA_site      2616..2629
                     /label=polya
     misc_feature    2589..2629
                     /label=BBa_B0012
     terminator      2589..2629
                     /label=BBa_B0012
     terminator      2501..2629
                     /label=BBa_B0015
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gattaaagag gagaaatact agatgaggaa tgacggaggc tttttgctgt ggtgggacgg
      121 tttgcgtagc gagatgcagc cgatccacga cagccagggc gtgttcgccg tcctggaaaa
      181 ggaagtgcgg cgcctgggct tcgattacta cgcctatggc gtgcgccaca cgattccctt
      241 cacccggccg aagaccgagg tccatggcac ctatcccaag gcctggctgg agcgatacca
      301 gatgcagaac tacggggccg tggatccggc gatcctcaac ggcctgcgct cctcggaaat
      361 ggtggtctgg agcgacagcc tgttcgacca gagccggatg ctctggaacg aggctcgcga
      421 ttggggcctc tgtgtcggcg cgaccttgcc gatccgcgcg ccgaacaatt tgctcagcgt
      481 gctttccgtg gcgcgcgacc agcagaacat ctccagcttc gagcgcgagg aaatccgcct
      541 gcggctgcgt tgcatgatcg agttgctgac ccagaagctg accgacctgg agcatccgat
      601 gctgatgtcc aacccggtct gcctgagcca tcgcgaacgc gagatcctgc aatggaccgc
      661 cgacggcaag agttccgggg aaatcgccat catcctgagc atctccgaga gcacggtgaa
      721 cttccaccac aagaacatcc agaagaagtt cgacgcgccg aacaagacgc tggctgccgc
      781 ctacgccgcg gcgctgggtc tcatctaata atactagaga ttaaagagga gaaatactag
      841 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      901 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      961 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     1021 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     1081 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     1141 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cttgcaggac
     1201 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1261 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1321 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     1381 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     1441 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     1501 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta ataatactag
     1561 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1621 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1681 ctgcgtttat atactagagt tgacggctag ctcagtccta ggtacagtgc tagctactag
     1741 agattaaaga ggagaaatac tagatgagga atgacggagg ctttttgctg tggtgggacg
     1801 gtttgcgtag cgagatgcag ccgatccacg acagccaggg cgtgttcgcc gtcctggaaa
     1861 aggaagtgcg gcgcctgggc ttcgattact acgcctatgg cgtgcgccac acgattccct
     1921 tcacccggcc gaagaccgag gtccatggca cctatcccaa ggcctggctg gagcgatacc
     1981 agatgcagaa ctacggggcc gtggatccgg cgatcctcaa cggcctgcgc tcctcggaaa
     2041 tggtggtctg gagcgacagc ctgttcgacc agagccggat gctctggaac gaggctcgcg
     2101 attggggcct ctgtgtcggc gcgaccttgc cgatccgcgc gccgaacaat ttgctcagcg
     2161 tgctttccgt ggcgcgcgac cagcagaaca tctccagctt cgagcgcgag gaaatccgcc
     2221 tgcggctgcg ttgcatgatc gagttgctga cccagaagct gaccgacctg gagcatccga
     2281 tgctgatgtc caacccggtc tgcctgagcc atcgcgaacg cgagatcctg caatggaccg
     2341 ccgacggcaa gagttccggg gaaatcgcca tcatcctgag catctccgag agcacggtga
     2401 acttccacca caagaacatc cagaagaagt tcgacgcgcc gaacaagacg ctggctgccg
     2461 cctacgccgc ggcgctgggt ctcatctaat aatactagag ccaggcatca aataaaacga
     2521 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2581 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//