LOCUS       BBa_K1159017            1630 bp    DNA     linear       12-Jun-2024
DEFINITION  Membrane-anchored FluA triple mutant in RFC[25] N-Part
ACCESSION   BBa_K1159017
VERSION     BBa_K1159017.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     CDS             107..628
                     /label=FluA triple mutant
     misc_feature    239..241
                     /label=Ala --> Ile
     misc_feature    389..391
                     /label=Arg --> Lys
     misc_feature    446..448
                     /label=Ser --> Thr
     CDS             107..628
                     /label=BBa_K1159003
     misc_feature    629..634
                     /label=25/25 scar
     misc_feature    629..634
                     /label=BBa_K1159993
     misc_feature    641..664
                     /label=''Strep''-tag II
     misc_feature    668..688
                     /label=TEV cleavage site
     misc_feature    635..691
                     /label=BBa_K1159309
     misc_feature    692..697
                     /label=25/25 scar
     misc_feature    692..697
                     /label=BBa_K1159993
     CDS             698..883
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    698..883
                     /label=BBa_K1159305
     misc_feature    884..889
                     /label=25/25 scar
     misc_feature    884..889
                     /label=BBa_K1159993
     misc_feature    890..913
                     /label=MiddleLinker
     misc_feature    890..913
                     /label=BBa_K243005
     misc_feature    914..919
                     /label=25/25 scar
     misc_feature    914..919
                     /label=BBa_K1159993
     CDS             920..1630
                     /label=GFPmut1
     CDS             920..1630
                     /label=BBa_K1159311
     CDS             698..1630
                     /label=BBa_K1159315
     CDS             635..1630
                     /label=BBa_K1159316
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcgacg tgtaccacga
      121 cggtgcctgt cccgaagtca agccagtcga caacttcgac tggtcccagt accatggtaa
      181 atggtgggaa gtcgccaaat accccagtcc caacgggaag tacggaaagt gcggatggat
      241 cgagtacact cctgaaggca agagtgtcaa agtttcgcgc tacgatgtaa tccacggcaa
      301 ggaatacttt atggaaggta ccgcctaccc agttggtgac tccaagattg gaaagatcta
      361 ccacagcagg actgttggag gttacaccaa aaagacggta ttcaacgtac tctccactga
      421 caacaagaac tacatcatcg gatacacgtg ccgctacgac gaggacaaga agggacactg
      481 ggaccatgtc tgggtgctct ccagaagcat ggtccttact ggtgaagcca agaccgctgt
      541 cgagaactac cttatcggct ccccagtagt cgactcccag aaactggtat acagtgactt
      601 ctctgaagcc gcctgcaagg tcaacaatac cggcagcgct tggtctcatc ctcaatttga
      661 aaagggtgaa aatctttatt ttcaaagcgg aaccggccct ggacaacctc cttttcctcc
      721 tcctcctcct tttacaccac ctcctccaca aactcctaac ggtgcttctg gtgagaactc
      781 tactggtgct attgctggtg gtgttgctgc tggtgcagct cttttgtttg ctgctcctgc
      841 tattggattc gcttggtgga gaagaagaag gcctattgag gctaccggcg gtggttctgg
      901 tggtggttct ggtaccggca gtaaaggaga agaacttttc actggagttg tcccaattct
      961 tgttgaatta gatggtgacg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1021 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1081 tccatggcca acacttgtca ctactctgac ttatggtgtt caatgctttt caagataccc
     1141 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     1201 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     1261 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1321 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1381 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1441 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttc
     1501 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1561 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1621 gctctacaaa
//