LOCUS       BBa_K1159018            2026 bp    DNA     linear       13-Jun-2024
DEFINITION  Membrane-anchored Catechol-2,3-dioxygenase (XylE) in RFC[25] N-Part
ACCESSION   BBa_K1159018
VERSION     BBa_K1159018.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     misc_feature    107..1024
                     /label=misc
     CDS             107..1024
                     /label=BBa_K648011
     CDS             1..1024
                     /label=BBa_K1159012
     misc_feature    1025..1030
                     /label=25/25 scar
     misc_feature    1025..1030
                     /label=BBa_K1159993
     misc_feature    1037..1060
                     /label=''Strep''-tag II
     misc_feature    1064..1084
                     /label=TEV cleavage site
     misc_feature    1031..1087
                     /label=BBa_K1159309
     misc_feature    1088..1093
                     /label=25/25 scar
     misc_feature    1088..1093
                     /label=BBa_K1159993
     CDS             1094..1279
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    1094..1279
                     /label=BBa_K1159305
     misc_feature    1280..1285
                     /label=25/25 scar
     misc_feature    1280..1285
                     /label=BBa_K1159993
     misc_feature    1286..1309
                     /label=MiddleLinker
     misc_feature    1286..1309
                     /label=BBa_K243005
     misc_feature    1310..1315
                     /label=25/25 scar
     misc_feature    1310..1315
                     /label=BBa_K1159993
     CDS             1316..2026
                     /label=GFPmut1
     CDS             1316..2026
                     /label=BBa_K1159311
     CDS             1094..2026
                     /label=BBa_K1159315
     CDS             1031..2026
                     /label=BBa_K1159316
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcaaca aaggtgtaat
      121 gcgaccgggc catgtgcagc tgcgtgtact ggacatgagc aaggccctgg aacactacgt
      181 cgagttgctg ggcctgatcg agatggaccg tgacgaccag ggccgtgtct atctgaaggc
      241 ttggaccgaa gtggataagt tttccctggt gctacgcgag gctgacgagc cgggcatgga
      301 ttttatgggt ttcaaggttg tggatgagga tgctctccgg caactggagc gggatctgat
      361 ggcatatggc tgtgccgttg agcagctacc cgcaggtgaa ctgaacagtt gtggccgccg
      421 cgtgcgcttc caggccccct ccgggcatca cttcgagttg tatgcagaca aggaatatac
      481 tggaaagtgg ggtttgaatg acgtcaatcc cgaggcatgg ccgcgcgatc tgaaaggtat
      541 ggcggctgtg cgtttcgacc acgccctcat gtatggcgac gaattgccag cgacctatga
      601 cctgttcacc aaggtgctcg gtttctatct ggccgaacag gtgctggacg aaaatggcac
      661 gcgcgtcgcc cagtttctca gtctgtcgac caaggcccac gacgtggcct tcattcacca
      721 tccggaaaaa ggccgcctcc atcatgtgtc cttccacctc gaaacctggg aagacttgct
      781 tcgcgccgcc gacctgatct ccatgaccga cacatctatc gatatcggcc caacccgcca
      841 cggcctcact cacggcaaga ccatctactt cttcgacccg tccggtaacc gcaacgaagt
      901 gttctgcggg ggagattaca actacccgga ccacaaacca gtgacctgga ccaccgacca
      961 gctgggcaag gcgatctttt accacgaccg cattctcaac gaacgattca tgaccgtgct
     1021 gaccaccggc agcgcttggt ctcatcctca atttgaaaag ggtgaaaatc tttattttca
     1081 aagcggaacc ggccctggac aacctccttt tcctcctcct cctcctttta caccacctcc
     1141 tccacaaact cctaacggtg cttctggtga gaactctact ggtgctattg ctggtggtgt
     1201 tgctgctggt gcagctcttt tgtttgctgc tcctgctatt ggattcgctt ggtggagaag
     1261 aagaaggcct attgaggcta ccggcggtgg ttctggtggt ggttctggta ccggcagtaa
     1321 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgacgttaa
     1381 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     1441 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     1501 tctgacttat ggtgttcaat gcttttcaag atacccagat catatgaaac agcatgactt
     1561 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     1621 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     1681 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     1741 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     1801 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     1861 acaaaatact ccaattggcg atggccctgt cctttcacca gacaaccatt acctgtccac
     1921 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     1981 tgtaacagct gctgggatta cacatggcat ggatgagctc tacaaa
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