LOCUS       BBa_K1159206            1447 bp    DNA     linear       26-May-2024
DEFINITION  Membrane-anchored SpyCatcher in RFC[25] N-Part
ACCESSION   BBa_K1159206
VERSION     BBa_K1159206.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     CDS             107..445
                     /label=N-terminal FbaB (modified SpyCatcher)
     misc_feature    206..208
                     /label=Iso --> Glu
     misc_feature    311..313
                     /label=Met --> Tyr
     misc_feature    197..199
                     /label=Reactive Lys for isopeptide forming reaction
     CDS             107..445
                     /label=BBa_K1159200
     CDS             1..445
                     /label=BBa_K1159204
     misc_feature    446..451
                     /label=25/25 scar
     misc_feature    446..451
                     /label=BBa_K1159993
     misc_feature    458..481
                     /label=''Strep''-tag II
     misc_feature    485..505
                     /label=TEV cleavage site
     misc_feature    452..508
                     /label=BBa_K1159309
     misc_feature    509..514
                     /label=25/25 scar
     misc_feature    509..514
                     /label=BBa_K1159993
     CDS             515..700
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    515..700
                     /label=BBa_K1159305
     misc_feature    701..706
                     /label=25/25 scar
     misc_feature    701..706
                     /label=BBa_K1159993
     misc_feature    707..730
                     /label=MiddleLinker
     misc_feature    707..730
                     /label=BBa_K243005
     misc_feature    731..736
                     /label=25/25 scar
     misc_feature    731..736
                     /label=BBa_K1159993
     CDS             737..1447
                     /label=GFPmut1
     CDS             737..1447
                     /label=BBa_K1159311
     CDS             515..1447
                     /label=BBa_K1159315
     CDS             452..1447
                     /label=BBa_K1159316
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcgttg ataccttatc
      121 aggtttatca agtgagcaag gtcagtccgg tgatatgaca attgaagaag atagtgctac
      181 ccatattaaa ttctcaaaac gtgatgagga cggcaaagag ttagctggtg caactatgga
      241 gttgcgtgat tcatctggta aaactattag tacatggatt tcagatggac aagtgaaaga
      301 tttctacctg tatccaggaa aatatacatt tgtcgaaacc gcagcaccag acggttatga
      361 ggtagcaact gctattacct ttacagttaa tgagcaaggt caggttactg taaatggcaa
      421 agcaactaaa ggtgacgctc atattaccgg cagcgcttgg tctcatcctc aatttgaaaa
      481 gggtgaaaat ctttattttc aaagcggaac cggccctgga caacctcctt ttcctcctcc
      541 tcctcctttt acaccacctc ctccacaaac tcctaacggt gcttctggtg agaactctac
      601 tggtgctatt gctggtggtg ttgctgctgg tgcagctctt ttgtttgctg ctcctgctat
      661 tggattcgct tggtggagaa gaagaaggcc tattgaggct accggcggtg gttctggtgg
      721 tggttctggt accggcagta aaggagaaga acttttcact ggagttgtcc caattcttgt
      781 tgaattagat ggtgacgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      841 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      901 atggccaaca cttgtcacta ctctgactta tggtgttcaa tgcttttcaa gatacccaga
      961 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1021 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1081 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1141 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1201 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1261 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tcctttcacc
     1321 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1381 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgagct
     1441 ctacaaa
//