LOCUS       BBa_K1159207            1147 bp    DNA     linear       27-May-2024
DEFINITION  Membrane-anchored SpyTag in RFC[25] N-Part
ACCESSION   BBa_K1159207
VERSION     BBa_K1159207.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     misc_feature    107..145
                     /label=SpyTag
     misc_feature    125..127
                     /label=Reactive Asp for isopeptide forming reaction
     CDS             107..145
                     /label=BBa_K1159201
     CDS             1..145
                     /label=BBa_K1159205
     misc_feature    146..151
                     /label=25/25 scar
     misc_feature    146..151
                     /label=BBa_K1159993
     misc_feature    158..181
                     /label=''Strep''-tag II
     misc_feature    185..205
                     /label=TEV cleavage site
     misc_feature    152..208
                     /label=BBa_K1159309
     misc_feature    209..214
                     /label=25/25 scar
     misc_feature    209..214
                     /label=BBa_K1159993
     CDS             215..400
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    215..400
                     /label=BBa_K1159305
     misc_feature    401..406
                     /label=25/25 scar
     misc_feature    401..406
                     /label=BBa_K1159993
     misc_feature    407..430
                     /label=MiddleLinker
     misc_feature    407..430
                     /label=BBa_K243005
     misc_feature    431..436
                     /label=25/25 scar
     misc_feature    431..436
                     /label=BBa_K1159993
     CDS             437..1147
                     /label=GFPmut1
     CDS             437..1147
                     /label=BBa_K1159311
     CDS             215..1147
                     /label=BBa_K1159315
     CDS             152..1147
                     /label=BBa_K1159316
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcgctc atattgtcat
      121 ggttgatgct tacaagccaa ctaagaccgg cagcgcttgg tctcatcctc aatttgaaaa
      181 gggtgaaaat ctttattttc aaagcggaac cggccctgga caacctcctt ttcctcctcc
      241 tcctcctttt acaccacctc ctccacaaac tcctaacggt gcttctggtg agaactctac
      301 tggtgctatt gctggtggtg ttgctgctgg tgcagctctt ttgtttgctg ctcctgctat
      361 tggattcgct tggtggagaa gaagaaggcc tattgaggct accggcggtg gttctggtgg
      421 tggttctggt accggcagta aaggagaaga acttttcact ggagttgtcc caattcttgt
      481 tgaattagat ggtgacgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      541 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      601 atggccaaca cttgtcacta ctctgactta tggtgttcaa tgcttttcaa gatacccaga
      661 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      721 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      781 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      841 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      901 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      961 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tcctttcacc
     1021 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1081 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgagct
     1141 ctacaaa
//