LOCUS       BBa_K1159209             628 bp    DNA     linear       18-May-2024
DEFINITION  Secretory nLuc_SpyTag fusion (IgKappa-SigP_nLuc_SpyTag) in RFC[25] 
            N-Part
ACCESSION   BBa_K1159209
VERSION     BBa_K1159209.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for P. patens
     CDS             5..67
                     /label=Ig Kappa Signal Peptide
     misc_feature    1..67
                     /label=BBa_K1159304
     misc_feature    68..73
                     /label=25/25 scar
     misc_feature    68..73
                     /label=BBa_K1159993
     CDS             74..583
                     /label=NanoLuc Luciferase
     CDS             74..583
                     /label=BBa_K1159001
     CDS             1..583
                     /label=BBa_K1159006
     misc_feature    584..589
                     /label=25/25 scar
     misc_feature    584..589
                     /label=BBa_K1159993
     misc_feature    590..628
                     /label=SpyTag
     misc_feature    608..610
                     /label=Reactive Asp for isopeptide forming reaction
     CDS             590..628
                     /label=BBa_K1159201
ORIGIN
        1 aacaatggaa accgatactc tcctcctctg ggttctcttg ctttgggttc caggatctac
       61 cggcgatacc ggcgtgttca ccctcgagga tttcgttgga gattggagac agaccgctgg
      121 atacaacctt gatcaggttt tggagcaggg tggtgtgtct agtcttttcc agaacctcgg
      181 agtgtctgtg acccctatcc agagaatcgt tctctctggt gagaacggac tcaagatcga
      241 tatccacgtg atcatccctt acgagggact ctcaggtgat cagatgggac agatcgagaa
      301 gattttcaag gtggtgtacc ctgttgatga tcaccacttc aaggtgatcc tccactacgg
      361 aactctcgtg atcgatggtg tgaccccaaa catgatcgat tacttcggaa ggccatacga
      421 gggaatcgct gtgttcgatg gaaagaagat taccgttacc ggaaccctct ggaacggaaa
      481 caagattatc gatgagaggc tcatcaaccc tgatggatct ctcttgttca gggtgaccat
      541 caacggtgtg actggttgga gactttgcga gagaatcctt gctaccggcg ctcatattgt
      601 catggttgat gcttacaagc caactaag
//