LOCUS       BBa_K1162015            1124 bp    DNA     linear       18-May-2024
DEFINITION  GFP with C-terminal 10x His-Tag
ACCESSION   BBa_K1162015
VERSION     BBa_K1162015.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=start
     CDS             227..943
                     /label=BBa_K1162011
     misc_feature    952..981
                     /label=10x-Histidine Tag
     misc_feature    982..984
                     /label=Stop
     misc_feature    985..987
                     /label=Stop
     misc_feature    952..987
                     /label=BBa_K844000
     stem_loop       1007..1050
                     /label=stem_loop
     misc_feature    996..1075
                     /label=BBa_B0010
     terminator      996..1075
                     /label=BBa_B0010
     stem_loop       1091..1110
                     /label=T7 TE
     misc_feature    1117..1117
                     /label=stop
     polyA_site      1111..1124
                     /label=polya
     misc_feature    1084..1124
                     /label=BBa_B0012
     terminator      1084..1124
                     /label=BBa_B0012
     terminator      996..1124
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctagcaaagg
      241 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      301 gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa agcttaccct
      361 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      421 ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt
      481 caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg
      541 gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga
      601 gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa
      661 ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa
      721 cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc attatcaaca
      781 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca
      841 atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt
      901 aactgctgct gggattacac atggcatgga tgagctctac aaatactaga gcatcatcac
      961 catcaccacc atcatcacca ttaataatac tagagccagg catcaaataa aacgaaaggc
     1021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1081 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//