LOCUS BBa_K1162306 2217 bp DNA linear 18-May-2024 DEFINITION Antimicrobial spider silk generator with C-terminus 10x His-Tag ACCESSION BBa_K1162306 VERSION BBa_K1162306.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 227..229 /label=Met CDS 230..340 /label=LL-37 CDS 227..340 /label=BBa_K1162006 misc_feature 349..432 /label=Elasticity Domain misc_feature 349..357 /label=Beta-helix misc_feature 358..372 /label=Beta-spiral misc_feature 379..393 /label=Beta-spiral misc_feature 394..408 /label=Beta-spiral misc_feature 417..432 /label=Beta-spiral misc_feature 433..516 /label=Elasticity Domain misc_feature 433..442 /label=Beta-helix misc_feature 443..458 /label=Beta-spiral misc_feature 462..477 /label=Beta-spiral misc_feature 478..492 /label=Beta-spiral misc_feature 501..516 /label=Beta-spiral misc_feature 517..533 /label=Linker Domain misc_feature 534..552 /label=Strength Domain CDS 349..552 /label=BBa_K844004 misc_feature 561..644 /label=Elasticity Domain misc_feature 561..569 /label=Beta-helix misc_feature 570..584 /label=Beta-spiral misc_feature 591..605 /label=Beta-spiral misc_feature 606..620 /label=Beta-spiral misc_feature 629..644 /label=Beta-spiral misc_feature 645..728 /label=Elasticity Domain misc_feature 645..654 /label=Beta-helix misc_feature 655..670 /label=Beta-spiral misc_feature 674..689 /label=Beta-spiral misc_feature 690..704 /label=Beta-spiral misc_feature 713..728 /label=Beta-spiral misc_feature 729..745 /label=Linker Domain misc_feature 746..764 /label=Strength Domain CDS 561..764 /label=BBa_K844004 misc_feature 773..856 /label=Elasticity Domain misc_feature 773..781 /label=Beta-helix misc_feature 782..796 /label=Beta-spiral misc_feature 803..817 /label=Beta-spiral misc_feature 818..832 /label=Beta-spiral misc_feature 841..856 /label=Beta-spiral misc_feature 857..940 /label=Elasticity Domain misc_feature 857..866 /label=Beta-helix misc_feature 867..882 /label=Beta-spiral misc_feature 886..901 /label=Beta-spiral misc_feature 902..916 /label=Beta-spiral misc_feature 925..940 /label=Beta-spiral misc_feature 941..957 /label=Linker Domain misc_feature 958..976 /label=Strength Domain CDS 773..976 /label=BBa_K844004 misc_feature 985..1068 /label=Elasticity Domain misc_feature 985..993 /label=Beta-helix misc_feature 994..1008 /label=Beta-spiral misc_feature 1015..1029 /label=Beta-spiral misc_feature 1030..1044 /label=Beta-spiral misc_feature 1053..1068 /label=Beta-spiral misc_feature 1069..1152 /label=Elasticity Domain misc_feature 1069..1078 /label=Beta-helix misc_feature 1079..1094 /label=Beta-spiral misc_feature 1098..1113 /label=Beta-spiral misc_feature 1114..1128 /label=Beta-spiral misc_feature 1137..1152 /label=Beta-spiral misc_feature 1153..1169 /label=Linker Domain misc_feature 1170..1188 /label=Strength Domain CDS 985..1188 /label=BBa_K844004 misc_feature 1197..1280 /label=Elasticity Domain misc_feature 1197..1205 /label=Beta-helix misc_feature 1206..1220 /label=Beta-spiral misc_feature 1227..1241 /label=Beta-spiral misc_feature 1242..1256 /label=Beta-spiral misc_feature 1265..1280 /label=Beta-spiral misc_feature 1281..1364 /label=Elasticity Domain misc_feature 1281..1290 /label=Beta-helix misc_feature 1291..1306 /label=Beta-spiral misc_feature 1310..1325 /label=Beta-spiral misc_feature 1326..1340 /label=Beta-spiral misc_feature 1349..1364 /label=Beta-spiral misc_feature 1365..1381 /label=Linker Domain misc_feature 1382..1400 /label=Strength Domain CDS 1197..1400 /label=BBa_K844004 misc_feature 1409..1492 /label=Elasticity Domain misc_feature 1409..1417 /label=Beta-helix misc_feature 1418..1432 /label=Beta-spiral misc_feature 1439..1453 /label=Beta-spiral misc_feature 1454..1468 /label=Beta-spiral misc_feature 1477..1492 /label=Beta-spiral misc_feature 1493..1576 /label=Elasticity Domain misc_feature 1493..1502 /label=Beta-helix misc_feature 1503..1518 /label=Beta-spiral misc_feature 1522..1537 /label=Beta-spiral misc_feature 1538..1552 /label=Beta-spiral misc_feature 1561..1576 /label=Beta-spiral misc_feature 1577..1593 /label=Linker Domain misc_feature 1594..1612 /label=Strength Domain CDS 1409..1612 /label=BBa_K844004 misc_feature 1621..1704 /label=Elasticity Domain misc_feature 1621..1629 /label=Beta-helix misc_feature 1630..1644 /label=Beta-spiral misc_feature 1651..1665 /label=Beta-spiral misc_feature 1666..1680 /label=Beta-spiral misc_feature 1689..1704 /label=Beta-spiral misc_feature 1705..1788 /label=Elasticity Domain misc_feature 1705..1714 /label=Beta-helix misc_feature 1715..1730 /label=Beta-spiral misc_feature 1734..1749 /label=Beta-spiral misc_feature 1750..1764 /label=Beta-spiral misc_feature 1773..1788 /label=Beta-spiral misc_feature 1789..1805 /label=Linker Domain misc_feature 1806..1824 /label=Strength Domain CDS 1621..1824 /label=BBa_K844004 misc_feature 1833..1916 /label=Elasticity Domain misc_feature 1833..1841 /label=Beta-helix misc_feature 1842..1856 /label=Beta-spiral misc_feature 1863..1877 /label=Beta-spiral misc_feature 1878..1892 /label=Beta-spiral misc_feature 1901..1916 /label=Beta-spiral misc_feature 1917..2000 /label=Elasticity Domain misc_feature 1917..1926 /label=Beta-helix misc_feature 1927..1942 /label=Beta-spiral misc_feature 1946..1961 /label=Beta-spiral misc_feature 1962..1976 /label=Beta-spiral misc_feature 1985..2000 /label=Beta-spiral misc_feature 2001..2017 /label=Linker Domain misc_feature 2018..2036 /label=Strength Domain CDS 1833..2036 /label=BBa_K844004 misc_feature 2045..2074 /label=10x-Histidine Tag misc_feature 2075..2077 /label=Stop misc_feature 2078..2080 /label=Stop misc_feature 2045..2080 /label=BBa_K844000 stem_loop 2100..2143 /label=stem_loop misc_feature 2089..2168 /label=BBa_B0010 terminator 2089..2168 /label=BBa_B0010 stem_loop 2184..2203 /label=T7 TE misc_feature 2210..2210 /label=stop polyA_site 2204..2217 /label=polya misc_feature 2177..2217 /label=BBa_B0012 terminator 2177..2217 /label=BBa_B0012 terminator 2089..2217 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tgctgggtga 241 ttttttccgc aaaagcaaag aaaaaatcgg caaagagttc aaacgtatcg ttcagcgcat 301 taaagatttt ctgcgtaatc tggttccgcg taccgaaagc tactagaggg tggatatggt 361 cctggagcag gtcaacaagg accaggtagt caaggacctg gttctggagg tcaacaagga 421 ccaggtggac aaggtggata tggtcctgga gcaggtcaac aaggaccagg tagtcaagga 481 cctggttctg gaggtcaaca aggaccaggt ggacaaggtc cttatggacc aagtgcagca 541 gcagcagcag catactagag ggtggatatg gtcctggagc aggtcaacaa ggaccaggta 601 gtcaaggacc tggttctgga ggtcaacaag gaccaggtgg acaaggtgga tatggtcctg 661 gagcaggtca acaaggacca ggtagtcaag gacctggttc tggaggtcaa caaggaccag 721 gtggacaagg tccttatgga ccaagtgcag cagcagcagc agcatactag agggtggata 781 tggtcctgga gcaggtcaac aaggaccagg tagtcaagga cctggttctg gaggtcaaca 841 aggaccaggt ggacaaggtg gatatggtcc tggagcaggt caacaaggac caggtagtca 901 aggacctggt tctggaggtc aacaaggacc aggtggacaa ggtccttatg gaccaagtgc 961 agcagcagca gcagcatact agagggtgga tatggtcctg gagcaggtca acaaggacca 1021 ggtagtcaag gacctggttc tggaggtcaa caaggaccag gtggacaagg tggatatggt 1081 cctggagcag gtcaacaagg accaggtagt caaggacctg gttctggagg tcaacaagga 1141 ccaggtggac aaggtcctta tggaccaagt gcagcagcag cagcagcata ctagagggtg 1201 gatatggtcc tggagcaggt caacaaggac caggtagtca aggacctggt tctggaggtc 1261 aacaaggacc aggtggacaa ggtggatatg gtcctggagc aggtcaacaa ggaccaggta 1321 gtcaaggacc tggttctgga ggtcaacaag gaccaggtgg acaaggtcct tatggaccaa 1381 gtgcagcagc agcagcagca tactagaggg tggatatggt cctggagcag gtcaacaagg 1441 accaggtagt caaggacctg gttctggagg tcaacaagga ccaggtggac aaggtggata 1501 tggtcctgga gcaggtcaac aaggaccagg tagtcaagga cctggttctg gaggtcaaca 1561 aggaccaggt ggacaaggtc cttatggacc aagtgcagca gcagcagcag catactagag 1621 ggtggatatg gtcctggagc aggtcaacaa ggaccaggta gtcaaggacc tggttctgga 1681 ggtcaacaag gaccaggtgg acaaggtgga tatggtcctg gagcaggtca acaaggacca 1741 ggtagtcaag gacctggttc tggaggtcaa caaggaccag gtggacaagg tccttatgga 1801 ccaagtgcag cagcagcagc agcatactag agggtggata tggtcctgga gcaggtcaac 1861 aaggaccagg tagtcaagga cctggttctg gaggtcaaca aggaccaggt ggacaaggtg 1921 gatatggtcc tggagcaggt caacaaggac caggtagtca aggacctggt tctggaggtc 1981 aacaaggacc aggtggacaa ggtccttatg gaccaagtgc agcagcagca gcagcatact 2041 agagcatcat caccatcacc accatcatca ccattaataa tactagagcc aggcatcaaa 2101 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2161 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //