LOCUS       BBa_K116404             1376 bp    DNA     linear       01-Jun-2024
DEFINITION  external phosphate sensing reporter
ACCESSION   BBa_K116404
VERSION     BBa_K116404.1  
FEATURES             Location/Qualifiers
     misc_feature    420..439
                     /label=PhoB binding site
     misc_feature    421..429
                     /label=-35
     misc_feature    448..455
                     /label=-10
     promoter        1..492
                     /label=BBa_K116401
     RBS             501..513
                     /label=RBS-3\Weak
     RBS             507..510
                     /label=RBS
     misc_feature    501..513
                     /label=BBa_B0032
     RBS             501..513
                     /label=BBa_B0032
     CDS             520..1239
                     /label=GFP protein
     CDS             520..1239
                     /label=BBa_E0040
     stem_loop       1259..1302
                     /label=stem_loop
     misc_feature    1248..1327
                     /label=BBa_B0010
     terminator      1248..1327
                     /label=BBa_B0010
     stem_loop       1343..1362
                     /label=T7 TE
     misc_feature    1369..1369
                     /label=stop
     polyA_site      1363..1376
                     /label=polya
     misc_feature    1336..1376
                     /label=BBa_B0012
     terminator      1336..1376
                     /label=BBa_B0012
     misc_feature    501..1376
                     /label=BBa_E0240
ORIGIN
        1 cacggtagta ttgaggaacg ccatgatatc gcgcgattca ttcagcgtgg cgaccgagtc
       61 atggtttcct gccagtacca ccagatgaca gccagtttgc tgtaaattga caacaaaacg
      121 gttgtataac gtgcgggcgt aactgggcgg cgagccggta tcgaaaacat caccggcaac
      181 aataatcgca tccacctgat gggtttgtgc tgtctccagc agccagtcaa gaaaagcctg
      241 atgttcagct tcgcggcttt tactgtagaa gttctggccg agatgccagt ctgaggtgtg
      301 aaggatgcgc ataacggttc cctggcgaaa aagcatgggc gcgattatac ccaaacagat
      361 gtgccatttg cttttttctg cgccacggaa atcaataacc tgaagatatg tgcgacgagc
      421 ttttcataaa tctgtcataa atctgacgca taatgacgtc gcattaatga tcgcaaccta
      481 tttattacaa catactagag tcacacagga aagtactaga tgcgtaaagg agaagaactt
      541 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      601 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      661 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      721 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      781 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      841 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      901 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      961 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     1021 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1081 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1141 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     1201 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     1261 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1321 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//