LOCUS BBa_K116530 6802 bp DNA linear 02-Jun-2024 DEFINITION cyanobacterial KaiABC oscillator with pOmpF as output ACCESSION BBa_K116530 VERSION BBa_K116530.1 FEATURES Location/Qualifiers misc_feature 27..88 /label=OmpR binding promoter 1..126 /label=BBa_K116500 RBS 135..147 /label=RBS-3\Weak RBS 141..144 /label=RBS misc_feature 135..147 /label=BBa_B0032 RBS 135..147 /label=BBa_B0032 CDS 154..873 /label=GFP protein CDS 154..873 /label=BBa_E0040 stem_loop 893..936 /label=stem_loop misc_feature 882..961 /label=BBa_B0010 terminator 882..961 /label=BBa_B0010 stem_loop 977..996 /label=T7 TE misc_feature 1003..1003 /label=stop polyA_site 997..1010 /label=polya misc_feature 970..1010 /label=BBa_B0012 terminator 970..1010 /label=BBa_B0012 misc_feature 1019..1037 /label=TetR 1 misc_feature 1019..1072 /label=BBa_R0040 promoter 1038..1043 /label=-35 misc_feature 1044..1062 /label=TetR 2 promoter 1061..1066 /label=-10 promoter 1019..1072 /label=BBa_R0040 misc_feature 1085..1088 /label=conserved RBS 1081..1092 /label=BBa_B0034 CDS 1099..1848 /label=BBa_K116501 stem_loop 1868..1911 /label=stem_loop misc_feature 1857..1936 /label=BBa_B0010 terminator 1857..1936 /label=BBa_B0010 stem_loop 1952..1971 /label=T7 TE misc_feature 1978..1978 /label=stop polyA_site 1972..1985 /label=polya misc_feature 1945..1985 /label=BBa_B0012 terminator 1945..1985 /label=BBa_B0012 misc_feature 1994..2012 /label=TetR 1 misc_feature 1994..2047 /label=BBa_R0040 promoter 2013..2018 /label=-35 misc_feature 2019..2037 /label=TetR 2 promoter 2036..2041 /label=-10 promoter 1994..2047 /label=BBa_R0040 misc_feature 2060..2063 /label=conserved RBS 2056..2067 /label=BBa_B0034 CDS 2074..3237 /label=BBa_K116502 stem_loop 3257..3300 /label=stem_loop misc_feature 3246..3325 /label=BBa_B0010 terminator 3246..3325 /label=BBa_B0010 stem_loop 3341..3360 /label=T7 TE misc_feature 3367..3367 /label=stop polyA_site 3361..3374 /label=polya misc_feature 3334..3374 /label=BBa_B0012 terminator 3334..3374 /label=BBa_B0012 misc_feature 3383..3401 /label=TetR 1 misc_feature 3383..3436 /label=BBa_R0040 promoter 3402..3407 /label=-35 misc_feature 3408..3426 /label=TetR 2 promoter 3425..3430 /label=-10 promoter 3383..3436 /label=BBa_R0040 misc_feature 3449..3452 /label=conserved RBS 3445..3456 /label=BBa_B0034 CDS 3465..4322 /label=BBa_J36801 stem_loop 4342..4385 /label=stem_loop misc_feature 4331..4410 /label=BBa_B0010 terminator 4331..4410 /label=BBa_B0010 stem_loop 4426..4445 /label=T7 TE misc_feature 4452..4452 /label=stop polyA_site 4446..4459 /label=polya misc_feature 4419..4459 /label=BBa_B0012 terminator 4419..4459 /label=BBa_B0012 CDS 4468..4555 /label=end of LacI coding region (inactive) misc_feature 4468..4667 /label=BBa_R0010 misc_feature 4556..4593 /label=CAP binding site promoter 4604..4609 /label=-35 promoter 4628..4633 /label=-10 misc_feature 4633..4667 /label=LacI binding site misc_feature 4640..4640 /label=start promoter 4468..4667 /label=BBa_R0010 misc_feature 4680..4683 /label=conserved RBS 4676..4687 /label=BBa_B0034 CDS 4694..5005 /label=BBa_J36802 CDS 5014..5101 /label=end of LacI coding region (inactive) misc_feature 5014..5213 /label=BBa_R0010 misc_feature 5102..5139 /label=CAP binding site promoter 5150..5155 /label=-35 promoter 5174..5179 /label=-10 misc_feature 5179..5213 /label=LacI binding site misc_feature 5186..5186 /label=start promoter 5014..5213 /label=BBa_R0010 misc_feature 5226..5229 /label=conserved RBS 5222..5233 /label=BBa_B0034 CDS 5240..6802 /label=BBa_J36804 ORIGIN 1 gacggtgttc acaaagttcc ttaaatttta cttttggtta catatttttt ctttttgaaa 61 ccaaatcttt atctttgtag cactttcacg gtagcgaaac gttagtttga atggaaagat 121 gcctgctact agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact 181 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc 241 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact 301 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa 361 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc 421 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt 481 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat 541 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat 601 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac 661 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc 721 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa 781 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt 841 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg 901 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 961 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc 1021 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag 1081 aaagaggaga aatactagat gaaaccccgc atcctcgtga tcgatgatga ctcagccatc 1141 ttggagctgg tcgccgtcaa tctggagatg tctggctatg acgtacgcaa agctgaggac 1201 ggcattaaag gtcaggcttt agctgttcag ctagttcccg acctgatcat gctggatcta 1261 atgctgccgc gggttgatgg ctttaccgtc tgtcagcgac tgcggcgcga tgagcgtact 1321 gccgaaattc cggtgctgat gctgaccgcc ctcggacaga ctcaggataa ggttgaaggc 1381 ttcaacgcgg gtgctgacga ttatctgact aagcccttcg aagttgaaga gatgctggcc 1441 cgcgtgcgtg ccttgctgcg gcgcaccgat cgcattcccc atgcagcccg ccatagcgaa 1501 attctcagct acggtccgct gaccctgatt cccgagcggt ttgaggccat ttggttcaac 1561 cgcacggtca agctgactca cttggaattt gagttgttgc actgcctgtt gcaacgccac 1621 ggccaaacgg ttgcgccgag cgaaatcctc aaagaagtct ggggctatga tcccgacgat 1681 gacatcgaga cgattcgcgt ccacatccgt catctgcgca ccaagctcga gcccgatccc 1741 cggcacccgc gctacatcaa aacggtctat ggagcgggct actgccttga gctgccggcc 1801 gagacggaac tccaccaaca cgccgatcag tttccttcgg cgtcctgata ctagagccag 1861 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1921 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1981 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2041 tgagcactac tagagaaaga ggagaaatac tagatgggag agtctctgtc accacaagca 2101 ctggctcaac cgttgctact gcaactgttc gtcgataccc ggcccctgtc acagcacatt 2161 gtgcagcggg ttaaaaatat tttggcagca gtagaggcaa ccgtccccat cagcttgcag 2221 gtgatcaatg tggcggacca gccacaactg gtggagtact accgcctagt cgtcacgcct 2281 gccctggtta aaattggtcc aggctctcgc caagttctga gtggcatcga cctcaccgat 2341 caattagcca accagttgcc ccagtggctg gttcagcaag aggccttttt tgccgatcga 2401 gagccacctg aagtcaacat tccgttcacg gagctaggcc aacccgagac ccccgcgttg 2461 cagcaggctg atgccttttt tcagcttcag caacaatacg ctgatctctc ggagcggaca 2521 aaattccttg agcaggtcat tgctctcgtc gcccacgacc tccgcaatcc gctaaccgct 2581 gctcttttgg cggtcgacac cattcaaatc cgcagtcaat ccttttctgt ggcgacagcc 2641 aaggaaatgc agggactgtg cagtctgttc gatcaggcac gatcgcaatt gcgtgaaatc 2701 gagcgcatga ttgccgagat tttggaggca actcgccact ctggcgaaag cttgcggatc 2761 aatccccgcg aagtcgtctt tgagccgctc ttacaacagg ttctggaaca gttgcatgaa 2821 cgctggcgga gtaagcagca gcagttgatc acggatgttc caggcgacct gcccacgctc 2881 tacgccgacc ctgaccgtct gcggcaggtt ctggtcaatc tgctagacaa cgccatcaaa 2941 tacactccgc ccgggggcac aatcacgatc gcagcgctcc atcgcacgag ccagaaagtg 3001 cagatcagca tcagcgacac gggctctggc atccctcgcg accagctcag cgtcattttc 3061 aagaacttgg tgcggctttc ccgcgatagc agccaagagg gctacggcat tggactatcg 3121 gtttgccagc gaattgttca ggcccacttt ggccgcatct gggttgcttc ggaactgggc 3181 caaggcagca ccttccactt cacgatgccg gtttatcgct acaccatgcc ctgctaatac 3241 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3301 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 3361 tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga 3421 tagagatact gagcactact agagaaagag gagaaatact agaggtgctc tcgcaaattg 3481 caatctgcat ttgggtggaa tcgacggcaa ttttgcagga ttgccagcgg gcgctgtcgg 3541 ccgatcgcta tcaactccaa gtctgtgagt ctggcgaaat gctcttggag tatgcccaaa 3601 cccatcgtga ccaaatcgac tgcctgattt tagtggcagc caatcccagc ttcagggcag 3661 ttgttcagca gctctgcttt gagggagtgg tggtaccagc gattgtcgta ggcgatcgcg 3721 acagtgagga tcccgatgaa ccagccaaag aacagctcta tcacagcgct gaactgcacc 3781 tcggtatcca tcagctcgag caattgccct accaagttga tgctgcactg gctgaatttc 3841 tgcgcttagc cccggtcgag accatggccg accacatcat gctgatgggg gccaaccacg 3901 atcccgagct atcgagccag cagcgggacc tcgctcagcg actacaagag cgcctaggct 3961 atctcggggt ctactacaag cgtgatcccg atcgctttct gcgcaaccta cccgcctacg 4021 aaagccaaaa gctgcaccaa gcgatgcaga ctagctatcg tgaaatcgtt ttgagctatt 4081 tttcgccgaa tagcaacctc aaccagagca ttgacaactt cgtcaacatg gctttctttg 4141 ccgatgttcc agtcaccaaa gtggtagaaa ttcacatgga gctgatggac gagtttgcca 4201 agaagctccg cgtagaggga cgttcagagg acattttgct ggattatcgg ctgactttaa 4261 ttgatgtaat tgcacatctt tgtgagatgt atcgacggtc tatcccacga gaaacctgat 4321 gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 4381 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 4441 gggcctttct gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc 4501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 4561 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 4621 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga 4681 ggagaaatac tagatgagcc ctcgtaaaac ctacattctc aagctctacg tcgccggcaa 4741 tactccaaac tcagtccgtg ccctcaaaac gctcaagaac attctcgaag ttgaatttca 4801 aggtgtttat gctctaaagg tgatcgatgt tctcaaaaat cctcagttgg cagaagagga 4861 taaaatccta gcgacgccaa ccctcgccaa ggttctacca ctgcctgtcc gacggattat 4921 tggtgattta tccgaccgtg agaaagtttt gattggcctt gatttactct acggcgaact 4981 tcaagattcc gacgacttct aataatacta gagcaatacg caaaccgcct ctccccgcgc 5041 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 5101 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 5161 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga 5221 gaaagaggag aaatactaga tgacttccgc tgagatgact agccctaata ataattctga 5281 gcaccaagcc atcgctaaga tgcgcacgat gattgaaggc tttgatgata ttagtcatgg 5341 cggtcttcca atcgggcgat cgaccctcgt tagtggtact tcaggaaccg gcaagaccct 5401 tttttctatt caatttctct ataacggtat tatcgagttt gatgagcctg gggttttcgt 5461 tactttcgaa gaaaccccgc aagatatcat taaaaacgcc cgtagttttg gctgggattt 5521 agccaagctg gtcgatgagg gcaaactatt tattcttgat gcttcacccg atccagaagg 5581 tcaagaggtt gttggcggct tcgatctctc tgctctgatt gagcggatta attatgcaat 5641 tcaaaagtat cgagcgcggc gggtttcaat tgactcggtc acgtccgttt tccagcaata 5701 tgatgcctct tctgtggttc gccgcgaact ctttcggttg gtagctcgcc taaaacaaat 5761 tggggcaact acggtcatga ccaccgagcg tatcgaggaa tatggcccga tcgctcgtta 5821 cggtgttgag gaatttgtct ccgataacgt cgtgattctc cgcaacgttt tggaagggga 5881 gcgccgtcgc cgcaccctcg aaatcctcaa gctacgtggc accagccaca tgaaagggga 5941 atatccgttc acgattacgg atcatggcat caatatcttc ccgctcgggg caatgcgcct 6001 tacgcagcga tcgtcgaacg tgcgtgtttc atctggtgtc gtccgactcg atgaaatgtg 6061 tggtgggggc ttctttaagg actcaatcat tctggcaact ggcgctacag gcactggtaa 6121 aactctgtta gttagccgtt tcgttgagaa tgcttgtgct aacaaagagc gggcgattct 6181 gttcgcttat gaagagtcac gagctcagct gctccgcaat gcctattcat ggggaatgga 6241 ctttgaggag atggagcgcc aaaacctcct caaaattgtt tgcgcctatc ctgaatccgc 6301 aggtcttgaa gaccatttgc agattattaa gtcggagatc aatgacttta agccagctcg 6361 tattgcaatc gactccctct ctgctttggc gcggggcgtt agcaacaatg ccttccgcca 6421 atttgtaatt ggtgtcactg gctacgcgaa acaagaagaa atcacgggac tattcacaaa 6481 taccagtgat caatttatgg gagcgcattc gattactgac tcccatatct caacaattac 6541 ggatacgatt atcttgctcc aatacgtcga gattcgtggc gaaatgtccc gcgccattaa 6601 cgtcttcaag atgcgcggat cttggcatga caaagcaatc cgcgagttca tgatcagcga 6661 caaagggccg gacatcaagg attctttccg gaactttgag cggattattt caggttcgcc 6721 aacacggatt accgtcgatg agaaaagcga actctcgcga attgtgcgcg gcgttcaaga 6781 aaaagggccg gagagctagt ag //